Effects of Fetal Growth Restriction on Postnatal Gut Microbiota in a Rat Model
Effects of Fetal Growth Restriction on Postnatal Gut Microbiota in a Rat Model
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DOI：
10.1097/mpg.0000000000003805
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发表时间：
2023-08-01
影响因子：
2.9
通讯作者：
Shimizu, Toshiaki
中科院分区：
文献类型：
--
作者：
Arai, Yoshiteru;Shoji, Hiromichi;Shimizu, Toshiaki
目的：胎儿生长受限（FGR）提示成年后生活方式相关疾病的风险增加。先前的研究表明，人类肠道生态失调与各种疾病之间存在关联。然而，研究FGR和肠道微生物群之间关系的报告很少。在此，我们假设FGR可能导致肠道生态失调，并通过限制妊娠期间母体蛋白质摄入来分析FGR大鼠模型中的肠道微生物群。方法：FGR组采用含7%蛋白质的饲料喂养妊娠Sprague道利大鼠至出生。对照组大鼠喂食21%的蛋白质。每周收集2-11周龄幼仔的粪便样本。从每个样品中提取DNA，并进行聚合酶链反应（PCR）扩增和测序。此外，在2周龄时分析盲肠中的短链脂肪酸，此时肠道微生物群的占有率存在差异。结果：肠道微生物群的比较分析仅在2周龄时显示出差异。对照组中疣微菌属明显更丰富（q < 0.1），而致病菌（包括肠球菌和肠杆菌科）在FGR组中倾向于增加。各组之间产生乙酸和丁酸的细菌的丰度也不同。与对照组相比，FGR组盲肠中的乙酸显著减少，而丁酸增加。结论：正常化FGR对出生后肠道微生物群的改变可能对宿主有有益的影响，因为FGR组导致肠道生态失调。
Objective:Fetal growth restriction (FGR) indicates increased risks of lifestyle-related diseases in adulthood. Previous studies showed the association between human gut dysbiosis and various diseases. However, reports examining the relationship between FGR and gut microbiota are scarce. Herein, we hypothesized that FGR may cause gut dysbiosis and analyzed the gut microbiota in a FGR rat model by restricting maternal protein intake during pregnancy. Methods:The FGR group was developed by feeding pregnant Sprague Dawley rats a diet containing 7% protein until birth. Control rats were fed 21% protein. Fecal samples of 2-11-week-old pups were collected weekly. DNA was extracted from each sample and subjected to polymerase chain reaction (PCR) amplification and sequencing. Additionally, short-chain fatty acids in the cecum were analyzed at 2 weeks of age, when there were differences in the occupancy of the gut microbiota. Results:Comparative analysis of the gut microbiota showed differences only at 2 weeks of age. Verrucomicrobia was significantly more abundant in the control group (q < 0.1), whereas pathogenic bacteria, including Enterococcus and Enterobacteriaceae, tended to increase in the FGR group. The abundance of acetic and butyric acid-producing bacteria also differed between groups. Acetic acid in the cecum was considerably decreased in the FGR group, while butyric acid was increased compared to that in the control group. Conclusions:Normalizing the alteration of FGR on postnatal gut microbiota may have beneficial effects for the host, since the FGR group caused gut dysbiosis.