Immunostimulatory DNA sequences function as T helper-1-promoting adjuvants
Immunostimulatory DNA sequences function as T helper-1-promoting adjuvants
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DOI：
10.1038/nm0897-849
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发表时间：
1997-08-01
期刊：
影响因子：
82.9
通讯作者：
Raz, E
中科院分区：
文献类型：
--
作者：
Roman, M;MartinOrozco, E;Raz, E
最近，一些短的细菌免疫刺激DNA序列(ISSS)的佐剂作用被认为是基于它们在基因免疫动物中刺激辅助性T细胞-1(Th1)反应的能力。我们在这里报道了非编码的、ISS富含的DNA或ISS寡核苷酸(ISS-ODN)能够有效地刺激对共同管理的抗原的免疫反应。ISS-DNA抑制IgE的合成，但促进免疫球蛋白和干扰素-γ的产生。此外，它们还启动干扰素-γ、干扰素-α、干扰素-β和白介素12和18的产生，所有这些都是有益的。Th1应答，增强细胞免疫。应考虑将非编码DNA佐剂添加到灭活或亚单位病毒疫苗中，这些疫苗本身只能提供部分感染保护。
An adjuvant role for certain short bacterial immunostimulatory DNA sequences (ISSs) has recently been proposed on the basis of their ability to stimulate T helper-1 (Th1) responses in gene-vaccinated animals. We report here that noncoding, ISS-enriched plasmid DNAs or ISS oligonucleotides (ISS-ODNs) potently stimulate immune responses to coadministered antigens. The ISS-DNAs suppress IgE synthesis, but promote IgG and interferon-gamma (IFN-gamma) production. They furthermore initiate the production of IFN-gamma, IFN-alpha, IFN-beta, and interleukins 12 and 18, all of which foster. Th1 responses and enhance cell-mediated immunity. Consideration should be given to adding noncoding DNA adjuvants to inactivated or subunit viral vaccines that, by themselves, provide only partial protection from infection.