Surface modification of neural probes with conducting polymer poly(hydroxymethylated-3,4-ethylenedioxythiophene) and its biocompatibility
Surface modification of neural probes with conducting polymer poly(hydroxymethylated-3,4-ethylenedioxythiophene) and its biocompatibility
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DOI：
10.1385/abab:128:2:117
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发表时间：
2006-02-01
影响因子：
3
通讯作者：
Shenai, M
中科院分区：
文献类型：
--
作者：
Xiao, YH;Martin, DC;Shenai, M
将一种新型导电聚合物聚（羟甲基化-3，4-乙撑二氧噻吩）（PEDOT-MeOH）电化学沉积到微机械神经探针的电极上。当掺杂聚苯乙烯磺酸盐时，从水溶液中获得均匀分布的膜。表面形貌粗糙，细胞粘附性好。阻抗谱表明，在10（0）到10（5）Hz的频率范围内，涂层电极的幅度低于裸金。由于神经探针和脑组织之间界面的生物相容性在探针植入中枢神经系统长期应用时起着重要作用，因此将生物分子掺入涂层中。九肽CDPGYIGSR作为导电膜中的抗衡物共沉积。涂层的表面形态是模糊的，提供了许多与神经细胞相互作用的生物活性位点。在生物相关频率1 kHz下，阻抗的幅度低至53 k Ω。体外实验表明，神经母细胞瘤细胞优先生长在PEDOT-MeOH/CDPGYIGSR涂层电极部位，并扩散到电极区域之外。
A novel conducting polymer, poly(hydroxymethylated-3,4-ethylenedioxythiophene) (PEDOT-MeOH), was electrochemically deposited onto the electrodes of micromachined neural probes. Uniformly distributed film was obtained from aqueous solution when doped with polystyrenesulfonate. The surface morphology was rough and had good cellular adhesion. Impedance spectroscopy showed that the magnitude of coated electrode was lower than that of the bare gold over a range of frequencies from 10(0), to 10(5) Hz. Since the biocompatibility of the interface between the neural probes and brain tissue plays an important role when the probes are implanted in the central nervous system for long-term application, biomolecules were incorporated into the coating. Nonapeptide CDPGYIGSR was codeposited as the counterion in the conducting films. The surface morphology of the coating was fuzzy, providing many bioactive sites for interaction with neural cells. The magnitude of impedance was as low as 53 k Omega at the biologically relevant frequency of 1 kHz. An in vitro experiment demonstrated that the neuroblastoma cells grew preferentially on the PEDOT-MeOH/CDPGYIGSR-coated electrode sites and spread beyond the electrode area.