Hypertrophy and Loss of Podocytes in Diabetic Nephropathy
Hypertrophy and Loss of Podocytes in Diabetic Nephropathy
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DOI：
10.2169/internalmedicine.48.2137
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发表时间：
2009-01-01
影响因子：
1.2
通讯作者：
Suzuki, Daisuke
中科院分区：
文献类型：
--
作者：
Miyauchi, Masaaki;Toyoda, Masao;Suzuki, Daisuke
目的足细胞的丢失在糖尿病肾病（DN）的发生和发展中起重要作用。虽然结构的变化，如足细胞肥大被认为是与足细胞损失，关系还没有得到彻底的调查，使用人DN renal tissues.Methods的主题是17例DN患者的肾活检病理组织学诊断。免疫组化检测足细胞标志物Wilm's tumor 1（WT 1）和synaptopodin（SPD），以确定足细胞的数量和评估足细胞肥大。足细胞数量与尿蛋白排泄量和系膜扩张程度呈负相关。足细胞肥大在糖尿病肾病患者中也较对照组明显。结论足细胞丢失和肥大参与了糖尿病肾病的发生和发展。
Objective The loss of podocytes has been reported to have a role in the onset and progression of diabetic nephropathy (DN). Although structural changes such as podocyte hypertrophy are considered to be associated with podocyte loss, the relationship has not been thoroughly investigated using human DN renal tissues.Methods The subjects were 17 patients with DN diagnosed histopathologically by renal biopsy. Immunostaining was performed with antibodies for Wilm's tumor 1 (WT1) and synaptopodin (SPD), which are markers of podocytes, to determine the number of podocytes and assess podocyte hypertrophy.Results The number of podocytes was decreased in DN patients compared with the controls. An inverse correlation was observed between the number of podocytes and both the urinary protein excretion and the extent of mesangial expansion. Podocyte hypertrophy was also more marked in DN patients compared with controls.Conclusion Based on these results, podocyte loss and hypertrophy were suggested to be involved in the development and progression of human DN.