SEQUENCE-ANALYSIS OF THE CLONED STREPTOCOCCAL CELL-SURFACE ANTIGEN-I/II
SEQUENCE-ANALYSIS OF THE CLONED STREPTOCOCCAL CELL-SURFACE ANTIGEN-I/II
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DOI：
10.1016/0014-5793(89)81632-1
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发表时间：
1989-11-20
期刊：
影响因子：
3.5
通讯作者：
LEHNER, T
中科院分区：
文献类型：
--
作者：
KELLY, C;EVANS, P;LEHNER, T
编码变异链球菌（Streptococcus mutons）C血清型（NG 5）Mr 185，000表面抗原（I/II）的基因P（以前命名为spaP 1）已被测序。该基因（4683 bp）编码1561个氨基酸残基的蛋白质，包括推定的信号肽（残基1-38）和跨膜（残基1537-1556）序列。N-末端区域（60-550）具有富含丙氨酸的重复序列，预计为α-螺旋。然而，C末端区域（800-1540）富含脯氨酸，有利于延伸结构。除了一个短的中央可变区的序列似乎是高度保守的S。变异血清型c分离的抗原I和抗原II多肽的N末端测序表明，前者代表完整抗原的N末端部分，后者代表完整抗原的C末端部分。
The genespa P(formerly designated asspaP1) encoding theMr185,000 surface antigen (I/II) ofStreptococcus mutons, serotype c (NG5), has been sequenced. The gene (4683 bp) encodes a protein of 1561 amino acid residues including putative signal peptide (residues 1–38) and transmembrane (residues 1537–1556) sequences. The N‐terminal region (60–550) has alanine‐rich repeats and is predicted to be α‐helical. However, the C‐ternunal region (800–1540) is proline‐rich and favours an extended structure. Except for a short central variable region the sequences appear to be highly conserved forS. mutansserotype c. N‐Tenninal sequencing of separated antigen I and antigen II polypeptides suggests that the former represents the N‐terminal and the latter the C‐terminal portions of the intact antigen.