Adsorption of bovine serum albumin to a polymer brush prepared by atom-transfer radical polymerization in a porous inorganic membrane
Adsorption of bovine serum albumin to a polymer brush prepared by atom-transfer radical polymerization in a porous inorganic membrane
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DOI：
10.1007/s10934-006-9031-0
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发表时间：
2007-12-01
影响因子：
2.6
通讯作者：
Tsuneda, Satoshi
中科院分区：
文献类型：
--
作者：
Kawakita, Hidetaka;Masunaga, Hiroyasu;Tsuneda, Satoshi
利用原子转移自由基聚合（ATRP）制备的聚合物刷在多孔无机膜上吸附蛋白质。用含卤素化合物对二氧化硅制备的多孔无机膜Shirasu多孔玻璃进行改性，使其结合活性物质进行聚合。在规定的时间内，用ATRP将卤素化合物聚合成甲基丙烯酸缩水甘油酯，然后进行化学改性。化学改性的进展使膜由于聚合物刷的附着而降低了多孔膜的磷酸盐缓冲通量。牛血清白蛋白（Bovine serum albumin， BSA）以12 mg / g的浓度通过聚合物刷附着的多孔膜被吸附。
Protein adsorption was performed by a polymer brush prepared by atom-transfer radical polymerization (ATRP) to a porous inorganic membrane. The porous inorganic membrane, Shirasu Porous Glass made from silica, was modified with a halogen-containing compound to bind the active species for the polymerization. Glycidyl methacrylate was polymerized from the halogen compound by ATRP for a prescribed time, and subsequently chemically modified. The progression of the chemical modification allowed the membrane to lower the phosphate-buffer flux of the porous membrane due to the attachment of the polymer brush. Bovine serum albumin (BSA), as a model protein, was adsorbed at 12 mg per gram of the membrane in permeating BSA solution through the polymer-brush-attached porous membrane.