Diaporthe from walnut tree (Juglans regia) in China, with insight of the Diaporthe eres complex
Diaporthe from walnut tree (Juglans regia) in China, with insight of the Diaporthe eres complex
复制标题
来自中国核桃树（Juglans regia）的毛叶枯，深入了解毛叶菊复合体
DOI：
10.1007/s11557-018-1395-4
复制
发表时间：
2018-07-01
影响因子：
2.4
通讯作者：
Tian, ChengMing
中科院分区：
文献类型：
--
作者：
Fan, XinLei;Yang, Qin;Tian, ChengMing
粉锈菌是一种重要的植物病原真菌，常见于多种寄主上。由于它们在形态上极其相似，以及令人困惑的多基因系统发育，因此相对较难识别，特别是在Diaporthe eres复合体中。本研究从中国核桃的病枝上采集了分离物。大多数菌株根据内部转录间隔区(ITS)、部分钙调蛋白(CAL)、组蛋白H3(His)、翻译延伸因子1-α(TEF1-α)和β-微管蛋白(Tub)基因聚为一类。为了关注这个复合体，进一步的系统发育分析中选择了CAL、TEF1-α和TUB，与结合的五基因系统发育相比，显示了更好的拓扑结构。结果表明，聚集在中国核桃核桃核孔复合体中的菌株均为核孔菌。结果提出了一个新的种分类标准。目前的研究发现了一种新的物种，在这里被描述为Diaporthe。蒂贝斯。
Species of Diaporthe are important plant pathogenic fungi that commonly occur on a wide range of hosts. They are relatively difficult to identify due to their extreme similarity in morphology and confusing multigene phylogeny, especially in the Diaporthe eres complex. In the present study, isolates were collected from diseased branches of Juglans regia in China. Most strains were clustered into the D. eres species complex based on the combined internal transcribed spacer (ITS) region, partial calmodulin (CAL), histone H3 (HIS), translation elongation factor 1-alpha (TEF1-alpha) and beta-tubulin (TUB) genes. To focus on this complex, CAL, TEF1-alpha and TUB were selected in further phylogenetic analyses that showed a better topology compared with combined five-gene phylogeny. Results revealed that all strains which clustered in the Diaporthe eres complex from Juglans regia in China were Diaporthe eres. Results suggested a revised species criterion in the Diaporthe eres complex. The current study uncovered a new species here described as Diaporthe. tibetensis.