Mutant studies of yeast phosphofructokinase.
Mutant studies of yeast phosphofructokinase.
复制标题
酵母磷酸果糖激酶的突变研究。
DOI：
10.1021/bi00537a037
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Fraenkel,DG
中科院分区：
文献类型：
--
作者：
Clifton,D;Fraenkel,DG
Drago Clifton和Dan G. Fraenkel*摘要：先前报道的磷酸果糖激酶突变体（pfkl-1突变）[Clifton， D, Weinstock， S. B, & Fraenkel， DG（1978）遗传学88,1-11]在葡萄糖上生长良好。我们在这里表明，在葡萄糖代谢过程中，它的单磷酸己糖水平很高，它产生正常的发酵产物，戊糖磷酸途径不是其主要的糖代谢途径，磷酸甘油激酶的额外阻滞导致果糖1,6 -二磷酸的积累，在某些情况下，抗原与野生型磷酸果糖激酶交叉反应。另一个磷酸果糖激酶突变株[Navon， G., Shulman， R. G., Yamane， T., Eccleshall， TR, Lam， K.-B]。[Baronofsky， JJ, & Marmur， J.(1979)Biochemistry 18, 4487-4499]在上述某些方面与pfkl-1突变体相似，但含有对果糖6-磷酸亲和力较低的磷酸果糖激酶，该酶已被纯化，是已知酶的突变形式。它的突变现在被称为pfk2- 1，与pfkl-1没有等位基因
Drago Clifton and Dan G. Fraenkel* abstract: A phosphofructokinase mutant (pfkl-1 mutation) previously reported [Clifton, D., Weinstock, S. B., & Fraenkel, DG (1978) Genetics 88, 1-11] grows well on glucose. We show here that its levels of hexose monophosphates are high during glucose metabolism, it produces normalfermentation products, the pentose phosphate pathway is not its major ca-tabolic route, an additional block in phosphoglyceratekinase causes accumulation of fructose 1, 6-bisphosphate, and antigen cross-reactive with wild-type phosphofructokinase is present in some conditions. Another phosphofructokinase mutant strain [Navon, G., Shulman, R. G., Yamane, T., Eccleshall, TR, Lam, K.-B., Baronofsky, JJ, & Marmur, J.(1979)Biochemistry 18, 4487-4499] resembles the pfkl-1 mutant in some of the above respects, but contains phosphofructokinase with lower affinity for fructose 6-phosphate, which has been purified and is a mutant form of the known enzyme. Its mutation, now called pfk2-l, is not allelic with pfkl-1, and