Growth of osteoclast precursor-like cells from whole mouse bone marrow: inhibitory effect of CSF-1.
Growth of osteoclast precursor-like cells from whole mouse bone marrow: inhibitory effect of CSF-1.
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小鼠全骨髓中破骨细胞前体样细胞的生长：CSF-1 的抑制作用。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
E. Burger
中科院分区：
文献类型：
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作者：
F. P. Wijngaert;M. C. Tas;J.W.M. van der Meer;E. Burger
我们研究了单核吞噬细胞（MP）和单核破骨细胞吞噬样（OCP样）细胞从新鲜分离的全小鼠骨髓的生长。抗酒石酸酸性磷酸酶（TRAcP）的表达可作为OCP样细胞鉴定的一般标志。骨吸收能力的培养细胞进行了研究，在共培养测定与骨膜游离胎儿骨雏形。新鲜分离的小鼠骨髓中每10（6）个有核骨髓细胞含有约30个OCP样细胞和1100个MP。OCP样细胞的数量没有增加，在悬浮培养含有巨噬细胞集落刺激因子（CSF-1），在MP的数量增加强烈。然而，OCP样细胞数量在单层培养中确实增加，这也允许骨髓成纤维细胞的贴壁依赖性生长。最强的OCP样细胞的增加发生在单层培养中的CSF-1的情况下。胎鼠皮肤成纤维细胞不增强OCP样细胞生长。OCP样细胞密度与无骨膜骨雏形共培养中形成的破骨细胞核数量密切相关。这些数据表明，单核细胞，细胞化学和功能类似破骨细胞前体，可以从小鼠骨髓生长。CSF-1抑制OCP样细胞的生长，表明破骨细胞前体细胞的生长要求不同于单核吞噬细胞。
We studied the growth of mononuclear phagocytes (MPs) and mononuclear osteoclast precursor-resembling (OCP-like) cells from freshly isolated whole mouse bone marrow. Expression of tartrate-resistant acid phosphatase (TRAcP) served as a general marker for the identification of OCP-like cells. Bone resorbing capacity of the cultured cells was studied in a coculture assay with periost-free fetal bone rudiments. Freshly isolated mouse bone marrow contained approximately 30 OCP-like cells and 1100 MPs per 10(6) nucleated bone marrow cells. OCP-like cell numbers did not increase in suspension cultures containing macrophage-colony stimulating factor (CSF-1), in contrast to the number of MPs which increased strongly. OCP-like cell numbers however did increase in monolayer cultures, which also allowed anchorage-dependent growth of bone marrow fibroblasts. Strongest increase of OCP-like cells occurred in monolayer cultures in the absence of CSF-1. Dermal fibroblasts of fetal mice did not enhance OCP-like cell growth. OCP-like cell density was strongly correlated with the number of osteoclast nuclei formed in cocultures with periost-free bone rudiments. These data indicate that mononuclear cells, cytochemically and functionally resembling osteoclast precursors, may be grown from mouse bone marrow. CSF-1 inhibited growth of OCP-like cells, indicating that osteoclast precursors differ from mononuclear phagocytes in growth requirements.