Prostaglandin biosynthesis by fat body from the tobacco hornworm, Manduca sexta.
Prostaglandin biosynthesis by fat body from the tobacco hornworm, Manduca sexta.
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烟草天蛾（Manduca sexta）脂肪体生物合成前列腺素。
DOI：
10.1016/0965-1748(94)90043-4
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发表时间：
1994
影响因子：
3.8
通讯作者：
Ogg,CL
中科院分区：
文献类型：
--
作者：
Stanley-Samuelson,DW;Ogg,CL
我们描述了前列腺素（PG）的生物合成微粒体丰富的制剂脂肪体从烟草天蛾幼虫。在大多数实验条件下合成了四种主要的PG，PGA 2、PGE 2、PGD 2和PGF 2 α。在大多数条件下，PGA 2是主要产物。与哺乳动物不同，在哺乳动物中，PGA 2通常被认为是由PGE 2的非酶促释放产生的，脂肪体制剂不将外源性PGE 2转化为PGA 2。这些发现表明，PGA 2是一种重要的脂肪体产物，其合成途径不涉及PGE 2。PG合成酶的活性和PG合成的整体概况是敏感的实验条件，包括孵育时间，温度和蛋白质浓度。最佳的PG生物合成与1毫克的微粒体丰富的蛋白质，在30°C下孵育1-2分钟。脂肪体制剂是敏感的两个非甾体抗炎药，吲哚美辛和萘普生，这两个抑制PG合成在低剂量。
We describe prostaglandin (PG) biosynthesis by microsomal-enriched preparations of fat body from larvae of the tobacco hornwormManduca sexta. Four major PGs were synthesized under most experimental conditions, PGA2, PGE2, PGD2and PGF2α. PGA2, was the predominant product under most conditions. Unlike mammals, in which PGA2, is generally thought to arise from non-enzymatic rearrangemets of PGE2, the fat body preparations did not convert exogenous PGE2into PGA2. These findings suggest that PGA2is an important fat body product that is synthesized by a route that does not involve PGE2. The PG synthase activity and the overall profile of PG synthesis were sensitive to experimental conditions, including incubation time, temperature, and protein concentration. Optimal PG biosynthesis was observed with 1 mg of microsomal-rich protein, incubated at 30°C for 1–2 min. The fat body preparation is sensitive to two non-steroidal anti-inflammatory drugs, indomethacin and naproxen, both of which inhibited PG synthesis at low dosages.