Distinct patterns of gene expression in the skin lesions of atopic dermatitis and psoriasis: A gene microarray analysis
Distinct patterns of gene expression in the skin lesions of atopic dermatitis and psoriasis: A gene microarray analysis
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DOI：
10.1016/j.jaci.2003.08.049
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发表时间：
2003-12-01
影响因子：
14.2
通讯作者：
Leung, DYM
中科院分区：
文献类型：
--
作者：
Nomura, L;Gao, B;Leung, DYM
背景：特应性皮炎（AD）和牛皮癣是两种最常见的慢性炎症性皮肤病。这两种疾病都有不同的临床表现和特定的炎症细胞浸润。以前的报告主要集中在两种皮肤病病变中的一个或两个基因或基因产物。然而，他们还没有捕捉到在这两种疾病的皮肤病变中诱导特异性细胞浸润所必须发生的复杂基因表达。DNA微阵列研究允许同时比较数千种信使rna，这些信使rna可以识别组织炎症反应的疾病特异性模式。目的：比较阿尔茨海默病与银屑病皮损的复杂基因表达谱。方法：从6例AD患者和7例银屑病患者的皮肤活检标本中提取RNA，用Hu-U95Av进行分析。GeneChip微阵列。为确认GeneChip结果，对所选基因进行实时PCR。结果：在AD皮肤中，CC趋化因子、CCL-13/MCP-4、CCL-18/PARC、CCL-27/CTACK等共18个基因的表达量与牛皮癣相比有统计学意义，表达量增加了约2倍。在银屑病皮肤中，CCL-4/ mip -1 β、CCL-20/ mip -3 α、CXCL-2/ gro - β、CXCL-8/IL-8、CXCR2/IL-8R等共62个基因的表达量较AD皮肤增加了约2倍。Real-time PCR证实了基因芯片的一些结果。结论：与银屑病相比，这些结果显示了AD中非常独特的基因表达模式，这可能解释了AD和银屑病的一些特征，包括在这些疾病中观察到的特异性炎症细胞浸润，即AD中的T(H)2细胞、嗜酸性粒细胞和肥大细胞，以及银屑病中的T(H)1细胞和中性粒细胞。这些观察结果可能有助于形成这两种皮肤病的特征性“特征”。
Background: Atopic dermatitis (AD) and psoriasis are the two most common chronic inflammatory skin diseases. Both of these diseases have distinct clinical findings and specific inflammatory cell infiltrates. Previous reports have focused individually on one or two genes or gene products in the lesions of both skin diseases. However, they have not captured the complex gene expression that must occur to induce specific cellular infiltrates in the skin lesions of these two diseases. DNA microarray studies allow the simultaneous comparison of thousands of messenger RNAs that may identify the disease-specific pattern of tissue inflammatory responses.Objective: To compare the complex gene expression pattern of AD versus psoriasis skin lesions.Methods: RNA was extracted from skin biopsy specimens of 6 patients with AD and 7 patients with psoriasis and analyzed with the use of Hu-U95Av.GeneChip microarrays. To confirm GeneChip results, real-time PCR of selected genes were performed. Results: In AD skin, a total of 18 genes including the CC chemokines, CCL-13/MCP-4, CCL-18/PARC, and CCL-27/CTACK showed a statistically significant, >2-fold increase of gene expression compared with psoriasis. In psoriasis skin, a total of 62 genes including CCL-4/MIP-1beta, CCL-20/MIP-3alpha, CXCL-2/GRO-beta CXCL-8/IL-8, and CXCR2/IL-8R showed a >2-fold increase of gene expression compared with AD skin. Real-time PCR confirmed several of these GeneChip results.Conclusions: These results show a very distinctive gene expression pattern in AD as compared with psoriasis that may explain several features of AD and psoriasis including the specific inflammatory cell infiltrates observed in these disorders, that is, T(H)2 cells, eosinophils, and mast cells in AD and T(H)1 cells and neutrophils in psoriasis. Such observations may contribute to a characteristic "signature" for these two skin diseases.