Proteomic Analysis of the Hyaloid Vascular System Regression during Ocular Development
Proteomic Analysis of the Hyaloid Vascular System Regression during Ocular Development
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DOI：
10.1021/pr800551m
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发表时间：
2008-11-01
影响因子：
4.4
通讯作者：
Azar, Dimitri T.
中科院分区：
文献类型：
--
作者：
Albe, Elena;Chang, Jin-Hong;Azar, Dimitri T.
我们描述了一种蛋白质组学的方法来研究差异蛋白质表达模式，并确定生理相关的血管生成和抗血管生成因子参与玻璃体血管系统的回归。差异表达的蛋白质进行了鉴定，使用二维凝胶电泳，然后通过纳米流色谱串联质谱。这些蛋白质有望为玻璃体血管系统的早期维护和最终退化提供深入了解。
We describe a proteomic approach to investigate the differential protein expression patterns and identify the physiologically relevant angiogenic and antiangiogenic factors involved in the hyaloid vascular system regression. Differentially expressed proteins were identified using two-dimensional gel electrophoresis followed by nanoflow chromatography coupled with tandem mass spectrometry. These proteins are expected to provide insight as to their function in the early maintenance and eventual regression of the hyaloid vascular system.