Olfactory nerve stimulation activates rat mitral cells via NMDA and non-NMDA receptors in vitro
Olfactory nerve stimulation activates rat mitral cells via NMDA and non-NMDA receptors in vitro
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DOI：
10.1097/00001756-199604100-00007
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发表时间：
1996-04-10
期刊：
影响因子：
1.7
通讯作者：
Shipley, MT
中科院分区：
文献类型：
--
作者：
Ennis, M;Zimmer, LA;Shipley, MT
用大鼠嗅球切片细胞外记录技术研究了哺乳动物嗅神经-僧帽细胞突触中介导兴奋性传递的神经递质和受体。单一的冲击施加到嗅神经引起短的潜伏期和延迟的兴奋反应在二尖瓣细胞。这两种反应都被阻断后浴应用犬尿烯酸，广谱谷氨酸受体拮抗剂，或DNQX，一个优先的非NMDA受体拮抗剂。特异性NMDA受体拮抗剂AP 5选择性地减弱延迟兴奋，但不减弱初始兴奋。这些结果表明，谷氨酸是哺乳动物嗅神经的主要兴奋性递质，并通过NMDA和非NMDA受体兴奋二尖瓣细胞。
THE neurotransmitter(s) and receptors mediating excitatory transmission at the mammalian olfactory nerve-mitral cell synapse were investigated using extracellular recordings in rat olfactory bulb slices. Single shocks applied to the olfactory nerve elicited both a short latency and a delayed excitatory response in mitral cells. Both responses were blocked after bath application of kynurenic acid, a broad-spectrum glutamate receptor antagonist, or DNQX, a preferential non-NMDA receptor antagonist. The specific NMDA receptor antagonist AP5 selectively attenuated the delayed, but not the initial excitation. These results suggest that glutamate is the major excitatory transmitter in the mammalian olfactory nerve, and excites mitral cells via NMDA and non-NMDA receptors.