Characteristics and capabilities of the Hyperspectral Imaging Microscope
Characteristics and capabilities of the Hyperspectral Imaging Microscope
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DOI：
10.1109/memb.2002.1032647
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发表时间：
2002-07-01
影响因子：
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通讯作者：
Garner, HR
中科院分区：
文献类型：
--
作者：
Huebschman, ML;Schultz, RA;Garner, HR
单通道多光谱成像使应用范围从全染色体绘画基因定位。我们已经开发了一个原型高光谱成像显微镜（HIM）工作站，以利用多光谱成像分析的一个单一的通过。我们用单次扫描的完整样品图像换取单次扫描的完整发射光谱。原型HI显微镜收集可见光发射光谱从显微镜载玻片的CCD相机的多次曝光，因为它跨载玻片的步骤。在每一步中，一个维度是空间，另一个是波长。该软件将空间和光谱信息与最少或不使用滤光片相关联，提供了比标准落射荧光显微镜更重要的优势。
Single-pass multispectral imaging enables applications ranging from whole-chromosome painting to gene mapping. We have developed a prototype Hyperspectral Imaging Microscope (HIM) Workstation to take advantage of multispectral imaging analysis on a single pass. We trade a full specimen image on a single pass for a full emission spectrum on a single pass. The prototype HI microscope collects the visible emission spectrum from a microscope slide by multiple exposures of the CCD camera as it steps across the slide. On each step, one dimension is space and the other is wavelength. The software correlates spatial and spectral information with minimal or no use of optical filters, offering important advantages over a standard epi-fluorescence microscope.