An olfactory bulb slice-based biosensor for multi-site extracellular recording of neural networks.
An olfactory bulb slice-based biosensor for multi-site extracellular recording of neural networks.
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DOI：
10.1016/j.bios.2011.01.005
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发表时间：
2011-03
影响因子：
12.6
通讯作者：
Qingmei Chen;Lidan Xiao;Qingjun Liu;S. Ling;Yifei Yin;Q. Dong;Ping Wang
中科院分区：
文献类型：
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作者：
Qingmei Chen;Lidan Xiao;Qingjun Liu;S. Ling;Yifei Yin;Q. Dong;Ping Wang
多点记录是神经网络研究的重要组成部分。为了研究嗅球神经网络的电生理特性，我们开发了一种新型的基于切片的多位点同步测量生物传感器。在本研究中，水平嗅球切片具有清晰的分层结构，作为传感元件，构建了基于微电极阵列的组织生物传感器。这种基于嗅球切片的生物传感器用于同时记录多个位置的细胞外电位。采用峰电位检测和互相关分析评价神经元的电生理活性。在嗅球切片的不同部位，自发电位和谷氨酸诱发电位呈现不同的电生理特征和放电模式。这种基于切片的生物传感器可以实现多位点同步监测，有利于搜索嗅球神经网络中的放电模式和突触连接。这也有助于进一步探讨嗅觉神经网络的嗅觉信息编码。
Multi-site recording is the important component for studies of the neural networks. In order to investigate the electrophysiological properties of the olfactory bulb neural networks, we developed a novel slice-based biosensor for synchronous measurement with multi-sites. In the present study, the horizontal olfactory bulb slices with legible layered structures were prepared as the sensing element to construct a tissue-based biosensor with the microelectrode array. This olfactory bulb slice-based biosensor was used to simultaneously record the extracellular potentials from multi-positions. Spike detection and cross-correlation analysis were applied to evaluate the electrophysiological activities. The spontaneous potentials as well as the induced responses by glutamic acid took on different electrophysiological characteristics and firing patterns at the different sites of the olfactory bulb slice. This slice-based biosensor can realize multi-site synchronous monitoring and is advantageous for searching after the firing patterns and synaptic connections in the olfactory bulb neural networks. It is also helpful for further probing into olfactory information encoding of the olfactory neural networks.