Preparations enriched for "immature" murine leukemia virus particles that remain in tissue culture fluids are deficient in Pr65gag proteolyic activity.
Preparations enriched for "immature" murine leukemia virus particles that remain in tissue culture fluids are deficient in Pr65gag proteolyic activity.
复制标题
保留在组织培养液中的富含“未成熟”鼠白血病病毒颗粒的制剂缺乏 Pr65gag 蛋白水解活性。
DOI：
10.1016/0042-6822(80)90559-0
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发表时间：
1980
期刊：
影响因子：
3.7
通讯作者：
R. Luftig
中科院分区：
文献类型：
--
作者：
Y. Yoshinaka;K. Ishigame;T. Ohno;S. Kageyama;K. Shibata;R. Luftig
Partially purified Moloney or Rauscher murine leukemia virus preparations obtained from 24-hr harvests of tissue culture fluids were separated into Pr65gagenriched and Pr65gagdeficient fractions by centrifugation on shallow sucrose or sucrose-D2O density gradients, e.g., 30 to 50% sucrose (w/v). The Pr65gagenriched fraction had a slightly higher relative density (ϱ = 1.18–1.20 g/cc) and a greatly increased number of “immature” virions (30%), than the Pr65gagdeficient fraction (ϱ = 1.16–1.18 g/cc; 2% immature virions). These two morphologically distinct virus populations were then further characterized as to the state of the RNA, the relative amount of exogenous reverse transcriptase activity, and the presence or absence of Pr65gagproteolytic activity. Both types of particles possessed 70 S RNA and similar amounts of exogenous reverse transcriptase activity. However, only the Pr65gagdeficient particles exhibited any Pr65gagproteolytic activity when incubated with Nonidet P-40 (2%, w/v), 10 mMDTT, and a Pr65gagsubstrate, i.e., immature virions. Thus it appears that the immature Rauscher and Moloney leukemia virions that remain in tissue culture fluids of chronically infected cells possess a cleavable Pr65gag, but are deficient in Pr65gagproteolytic activity.
