Assessment of Photosynthetic Dysfunction in a Whole Tomato Plant with Chlorophyll Fluorescence Induction Imaging
Assessment of Photosynthetic Dysfunction in a Whole Tomato Plant with Chlorophyll Fluorescence Induction Imaging
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用叶绿素荧光感应成像评估整个番茄植株的光合功能障碍
DOI：
10.2525/ecb.48.151
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
H. Nishina
中科院分区：
文献类型：
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作者：
K. Takayama;Yoshiaki Sakai;T. Oizumi;H. Nishina
叶绿素荧光是由叶绿素发出的红光，是一种色素，因此准确测量这种发射使我们能够评估植物的光合作用功能。叶绿素荧光诱导成像测量了一种叶绿素荧光诱导现象。这种现象是在黑暗条件下用稳定强度的激发光照射植物体内而引起的叶绿素荧光强度的动态变化。在此过程中，叶绿素荧光强度的时间过程称为诱导曲线。在这项研究中，我们开发了一个完整的番茄植株的叶绿素荧光诱导成像系统，作为温室植物诊断的第一个原型。利用该系统研究了PPFD1500μ−2s−1光照2.5h对1.1m高番茄植株光合作用的影响。诱导曲线在处理前和处理后有明显的变化，但在黑暗条件下6h即可恢复。在恢复过程中，作为感应曲线的特征拐点的P和M的拐点表现出不同的行为。在光照处理后1.5h内，尽管P已基本恢复，但M仍维持在较低水平。这一结果表明，叶绿素荧光诱导成像，特别是对P和M拐点图像的同时监测，对于检测整个植物水平上看不见的光合作用障碍是有用的。
Chlorophyll fluorescence is red light emitted from chlorophyll a pigment and hence accurate measurement of this emission allows us to assess photosynthetic functions of the plant. The chlorophyll fluorescence induction imaging measures a chlorophyll fluorescence induction phenomenon. This phenomenon is a dynamic change in chlorophyll fluorescence intensity induced by illuminating plant body with an excitation light at a stable intensity under dark condition. The time course of the chlorophyll fluorescence intensity during this phenomenon is called induction curve. In this study, we developed a chlorophyll fluorescence induction imagining system for whole tomato plants as a first prototype for plant diagnosis in greenhouse. By using this system, we assessed the effects of sunlight exposure treatment, i.e. PPFD 1500 μmol m−2s−1 for 2.5 h, on the photosynthetic functions of a whole tomato plant of 1.1 m high. A substantial transformation of induction curve was observed between before and just after the treatment, but it was recovered in 6 h under dark condition. During the recovery process, the inflection points of P and M, which are the characteristic inflections of an induction curve, showed different behaviour. The M kept lower values for 1.5 h after the sunlight exposure treatment even though the P had been almost recovered. This result suggests that the chlorophyll fluorescence induction imaging, especially concurrent monitoring of the images of P and M inflection points, is useful to detect invisible photosynthetic dysfunctions at whole plant level.