Using segmental isotope analysis of teleost fish vertebrae to estimate trophic discrimination factors of bone collagen

Using segmental isotope analysis of teleost fish vertebrae to estimate trophic discrimination factors of bone collagen
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利用硬骨鱼椎骨的节段同位素分析来估计骨胶原的营养区分因子

DOI:
10.1002/lom3.10298
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发表时间:
2018
期刊:
Limnology and Oceanography: Methods
影响因子:
--
通讯作者:
Tayasu Ichiro
Tayasu Ichiro
中科院分区:
--
文献类型:
--
作者:
Matsubayashi Jun;Umezawa Yu;Matsuyama Michiya;Kawabe Ryo;Mei Weiping;Wan Xin;Shimomae Atsushi;Tayasu Ichiro

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采用回顾性同位素分析方法,对沙丁鱼(Sardinops melanostictus)和牙鲆(Paralichthys olivaceus)的骨胶原营养判别因子(TDF)进行了研究。沙丁鱼和日本比目鱼分别饲喂9个月和4个月的单一饲料。在实验结束时,我们从六条沙丁鱼和七条日本比目鱼中提取了椎体,并将其细分为多个部分。提取各节段的骨胶原,测定各节段和食物来源的δ 13 C和δ 15 N。在所有鱼样中,骨胶原的δ 13 C和δ 15 N值从椎骨中心向边缘逐渐增加。这一趋势与这些鱼类饮食中预测的同位素变化一致。沙丁鱼的δ 13 C和δ 15 N的TDFs分别为5.58 ± 0.25‰和−0.70 ± 0.25‰;日本比目鱼的δ 13 C和δ 15 N的TDFs分别为3.89 ± 0.14‰和1.18 ± 0.35‰。我们的研究结果表明,椎骨的节段分析可以重建两种鱼类多个生命阶段的δ 13 C和δ 15 N值,并有助于估计TDFs和鱼骨胶原蛋白的周转时间。
We conducted feeding experiments with sardine (Sardinops melanostictus) and Japanese flounder (Paralichthys olivaceus) to investigate trophic discrimination factors (TDFs) in bone collagen using retrospective isotope analysis. Sardines and Japanese flounder were fed a single diet for 9 months and 4 months, respectively. At the end of the experiments, we extracted vertebral centra from six sardines and seven Japanese flounder and subdivided them into multiple sections. We extracted bone collagen from each section, and measured theδ13C andδ15N of each vertebral section and food source. In all fish samples, theδ13C andδ15N values of bone collagen increased from the centers of vertebrae to the marginal sections. This trend was consistent with the predicted isotopic shift in the diets of these fishes. The TDFs for sardines were estimated at 5.58 ± 0.25‰ (mean ± SD) forδ13C and −0.70 ± 0.25‰ forδ15N; those for Japanese flounder were 3.89 ± 0.14‰ forδ13C and 1.18 ± 0.35‰ forδ15N. Our results show that segmental analysis of vertebrae can reconstructδ13C andδ15N values for multiple life‐stages of both fish species and is useful for estimating TDFs and turnover times of fish bone collagen.
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