Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin
Targeted induction of a silent fungal gene cluster encoding the bacteria-specific germination inhibitor fumigermin
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DOI：
10.7554/elife.52541
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发表时间：
2020-02-21
期刊：
影响因子：
7.7
通讯作者：
Brakhage, Axel A.
中科院分区：
文献类型：
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作者：
Stroe, Maria Cristina;Netzker, Tina;Brakhage, Axel A.
微生物产生大量具有多种生物活性的次生代谢物。它们的许多编码基因簇在标准的实验室条件下是沉默的，因为它们的激活需要生态环境，如其他微生物的存在。大多数SMS的真正生态功能仍不清楚，但了解沉默基因簇的激活和产生的化合物的生态功能对于揭示微生物群中的功能相互作用具有重要意义。在这里，我们报告了真菌烟曲霉的一个尚未鉴定的沉默基因簇，它在细菌-真菌相互作用过程中被雷帕霉素链霉菌激活。由此产生的天然产物是新的真菌代谢物烟雾剂，其生物合成需要聚酮合成酶FgnA。Fumigermin抑制诱导雷帕霉素链霉菌孢子的萌发，从而帮助这种真菌保护共享栖息地的资源不受细菌竞争对手的攻击。
Microorganisms produce numerous secondary metabolites (SMs) with various biological activities. Many of their encoding gene clusters are silent under standard laboratory conditions because for their activation they need the ecological context, such as the presence of other microorganisms. The true ecological function of most SMs remains obscure, but understanding of both the activation of silent gene clusters and the ecological function of the produced compounds is of importance to reveal functional interactions in microbiomes. Here, we report the identification of an as-yet uncharacterized silent gene cluster of the fungus Aspergillus fumigatus, which is activated by the bacterium Streptomyces rapamycinicus during the bacterial-fungal interaction. The resulting natural product is the novel fungal metabolite fumigermin, the biosynthesis of which requires the polyketide synthase FgnA. Fumigermin inhibits germination of spores of the inducing S. rapamycinicus, and thus helps the fungus to defend resources in the shared habitat against a bacterial competitor.