A SIGNAL SEQUENCE IS NOT SUFFICIENT TO LEAD BETA-GALACTOSIDASE OUT OF THE CYTOPLASM
A SIGNAL SEQUENCE IS NOT SUFFICIENT TO LEAD BETA-GALACTOSIDASE OUT OF THE CYTOPLASM
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DOI：
10.1038/286356a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
SCHWARTZ, M
中科院分区：
文献类型：
--
作者：
MORENO, F;FOWLER, AV;SCHWARTZ, M
构建了大肠杆菌菌株，其中lacZ（细胞质酶β-半乳糖苷酶的基因）与lamB（外膜蛋白的基因）融合。一种这样的菌株产生β-半乳糖苷酶，尽管其在氨基末端具有lamB蛋白前体的完整信号序列，但该酶仍保留在细胞质中。
Escherichia coli strains were constructed in which lacZ, the gene for the cytoplasmic enzyme .beta.-galactosidase, is fused to lamB, the gene for an outer membrane protein. One such strain produces a .beta.-galactosidase which remains cytoplasmic even though it possesses the complete signal sequence of the lamB protein precursor at the amino-terminal end.