Polyunsaturated fatty acid supplements modulate mast cell membrane microdomain composition
Polyunsaturated fatty acid supplements modulate mast cell membrane microdomain composition
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DOI：
10.1016/j.cellimm.2012.03.004
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发表时间：
2012-01-01
影响因子：
4.3
通讯作者：
Schumann, Julia
中科院分区：
文献类型：
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作者：
Basiouni, Shereen;Stoeckel, Katja;Schumann, Julia
在本研究中，分析了犬肥大细胞瘤细胞系（C2）的脂筏组成。使用无洗涤剂的分离技术将脂筏与非筏质膜很好地分离。为了研究与非筏细胞膜相比，n-3和n-6多不饱和脂肪酸（PUFA）对筏脂肪酸组成的影响，C2补充有以下之一：α-亚麻酸、二十碳五烯酸、二十二碳六烯酸、亚油酸或花生四烯酸。用特定的PUFA富集培养基导致筏和非筏膜中这种脂肪酸的含量增加。胆固醇和蛋白质的含量被认为是不受影响的脂肪酸谱的变化。总之，我们的数据提供了强有力的证据表明，多不饱和脂肪酸调节肥大细胞膜微区的脂质组成和生理特性，从而可能对肥大细胞功能产生影响。(C)2012 Elsevier Inc. All rights reserved.
In the present study, the lipid raft composition of a canine mastocytoma cell line (C2) was analyzed. Lipid rafts were well separated from non-raft plasma membranes using a detergent-free isolation technique. To study the influence of n-3 and n-6 polyunsaturated fatty acids (PUFA) on raft fatty acid composition in comparison to non-raft cell membrane, C2 were supplemented with one of the following: alpha-linolenic acid, eicosapentaenoic acid, docosahexaenoic acid, linoleic acid or arachidonic acid. Enrichment of the culture medium with a specific PUFA resulted in an increase in the content of this fatty acid both in rafts and non-raft membranes. Contents of cholesterol and protein were found not to be affected by the changes in the fatty acid profiles. In conclusion, our data provide strong evidence that PUFA modulate lipid composition and physiological properties of membrane micro domains of mast cells which in turn may have effects on mast cell function. (C) 2012 Elsevier Inc. All rights reserved.