Cellular and molecular comparison of redifferentiation of intramuscular- and visceral-adipocyte derived progeny cells.
Cellular and molecular comparison of redifferentiation of intramuscular- and visceral-adipocyte derived progeny cells.
复制标题
肌内脂肪细胞和内脏脂肪细胞衍生的子代细胞再分化的细胞和分子比较
DOI：
10.7150/ijbs.6.80
复制
发表时间：
2010-01-21
影响因子：
9.2
通讯作者：
Jiang Z
中科院分区：
文献类型：
--
作者：
Chen J;Dodson MV;Jiang Z
在本研究中，从猪内脏和肌内脂肪库中分离、纯化成熟脂肪细胞，并使其在体外进行去分化和再分化。在第1、2、4、6和8天的再分化过程中，我们观察到内脏和肌内脂肪衍生的后代细胞具有相似的积累脂质的能力。然而，在第10、12、14和16天，后者的后代细胞积累脂质的速度要快得多--在第16天含量几乎翻了一番（P < 0.05）。肌内脂肪来源的后代细胞中脂质积累的这种更快潜力随后得到所有这9个时间点的CCAAT/增强子结合蛋白-α（CEBP-α）和过氧化物酶体增殖物激活受体-γ（PPAR-γ）以及二酰基甘油O-酰基转移酶同系物1（DGAT 1）的更高表达的支持，脂肪酸结合蛋白4（FABP 4）和脂肪酸合成酶（FABP 4）在某些时间点的表达水平显著高于对照组（P < 0.05）。这些初步数据表明，脂肪库的差异存在于纯化的脂肪谱系细胞的能力，再分化和形成活的脂质同化细胞在体外。因此，我们目前的研究可能为开发生物医学和农业应用工具以及确定脂肪谱系的储存特异性细胞的调节提供基础。更多动物的进一步研究将验证和扩展我们的结果。
In the present study, mature adipocytes from pig-derived visceral and intramuscular adipose depots were isolated, purified, and allowed to undergo dedifferentiation and redifferentiation in vitro. During the redifferentiation process at days 1, 2, 4, 6, and 8, we observed that both visceral- and intramuscular adipose-derived progeny cells possessed a similar capacity to accumulate lipid. However, at days 10, 12, 14, and 16, the latter progeny cells accumulated lipid much faster--the content almost doubled at day 16 (P < 0.05). Such faster potential of lipid accumulation in the intramuscular adipose-derived progeny cells was then supported by higher expressions of CCAAT/enhancer binding protein-α (CEBP-α) and peroxisome proliferator-activated receptor-γ (PPAR-γ) at all these nine time points, and diacylglycerol O-acyltransferase homolog 1 (DGAT1), fatty acid binding protein 4 (FABP4) and fatty acid synthase (FASN) at some time points (P < 0.05). These preliminary data suggest that adipose depot differences exist with respect to ability of purified cells of the adipose lineage to redifferentiate and form viable lipid-assimilating cells in vitro. Therefore, our present study might provide a foundation to develop tools for biomedical and agricultural applications, as well as to determine the regulation of depot-specific cells of the adipose lineage. Further studies with more animals will validate and expand our results.