A light-up probe targeting for Bcl-2 2345 G-quadruplex DNA with carbazole TO
A light-up probe targeting for Bcl-2 2345 G-quadruplex DNA with carbazole TO
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带有咔唑 TO 的 Bcl-2 2345 G 四链体 DNA 发光探针
DOI：
10.1016/j.saa.2017.10.012
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发表时间：
2018-02-15
影响因子：
4.4
通讯作者：
Zhou, Jianguo
中科院分区：
文献类型：
--
作者：
Gu, Yingchun;Lin, Dayong;Zhou, Jianguo
选择性识别具有特定结构和功能的g -四重体在生物化学和药物化学中具有重要意义。咔唑衍生物是一类具有许多优良光学性能的荧光探针。在本研究中，染料（咔唑TO）在bcl-2 2345 G4的存在下，其荧光比在几乎没有荧光的水缓冲条件下增加了近70倍，比在其他dna存在下的荧光增加了10-30倍。通过抑制G4/Hemin过氧化物酶活性实验、核磁共振滴定和分子对接模拟的结合研究结果表明，bcl-2 2345对G4的高亲和力和选择性源于其与g -四重奏的端叠相互作用。该方法灵敏度高，选择性好，反应速度快，可用于bcl-2 2345 g四联体的抗肿瘤识别或抗肿瘤药物。(C) 2017 Elsevier B.V.版权所有
As its significant role, the selective recognition of G-quadruplex with specific structures and functions is important in biological and medicinal chemistry. Carbazole derivatives have been reported as a kind of fluorescent probe with many excellent optical properties. In the present study, the fluorescence of the dye (carbazole TO) increased almost 70 fold in the presence of bcl-2 2345 G4 compared to that alone in aqueous buffer condition with almost no fluorescence and 10-30 fold than those in the presence of other DNAs. The binding study results by activity inhibition of G4/Hemin peroxidase experiment, NMR titration and molecular docking simulation showed the high affinity and selectivity to bcl-2 2345 G4 arises from its end-stacking interaction with G-quartet. It is said that a facile approach with excellent sensitive, good selectivity and quick response for bcl-2 2345 G-quadruplex was developed and may be used for antitumor recognition or antitumor agents. (C) 2017 Elsevier B.V. All rights reserved.