Rack1, a receptor for activated protein kinase C, interacts with integrin β subunit
Rack1, a receptor for activated protein kinase C, interacts with integrin β subunit
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DOI：
10.1074/jbc.273.4.2379
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发表时间：
1998-01-23
影响因子：
4.8
通讯作者：
Chang, DD
中科院分区：
文献类型：
--
作者：
Liliental, J;Chang, DD
整合素β亚基胞质结构域对于激活依赖性细胞粘附和粘附依赖性信号传导事件很重要。我们报告了整合素β亚基胞质结构域和Rack1之间的相互作用，Rack1是一种色氨酸-天冬氨酸（WD）重复蛋白，已被证明可以结合活化的蛋白激酶C，整合素β（2）亚基上的Rack1结合位点位于保守的膜近端区域内。在酵母双杂交测定中，WD 重复 5 到 7 个 Rack1 (Rack1-WD5/7) 与整合素 beta(1)、beta(2) 和 beta(5) 细胞质结构域相互作用。在真核细胞中，Rack1 与至少两种不同的 β 整合素、293T 细胞中的 β(1) 整合素和 JY 中的 β(1) 整合素进行免疫共沉淀。 在淋巴母细胞中，虽然Rack1-WD5/7组成性地结合整合素，但全长Rack1在体内与整合素的结合需要佛波酯的处理，从而促进细胞铺展和粘附。这些发现表明Rack1可能将蛋白激酶C直接与整合素连接并参与整合素功能的调节。
The integrin beta subunit cytoplasmic domains are important for activation-dependent cell adhesion and adhesion-dependent signaling events. We report an interaction between integrin beta subunit cytoplasmic domain and Rack1, a Trp-Asp (WD) repeat protein that has been shown to bind activated protein kinase C, The Rack1-binding site on integrin beta(2) subunit resides within a conserved, membrane proximal region. In the yeast two-hybrid assay, WD repeats five to seven of Rack1 (Rack1-WD5/7) interact with integrin beta(1), beta(2), and beta(5) cytoplasmic domain, In eukaryotic cells, Rack1 co-immunoprecipitates with at least two different beta integrins, beta(1) integrins in 293T cells and beta(1) integrins in JY lymphoblastoid cells, Whereas Rack1-WD5/7 binds integrins constitutively, the association of full length Rack1 to integrins in vivo requires a treatment with phorbol esters, which promotes cell spreading and adhesion, These findings suggest that Rack1 may link protein kinase C directly to integrins and participate in the regulation of integrin functions.