Transport of IRW, an Ovotransferrin-Derived Antihypertensive Peptide, in Human Intestinal Epithelial Caco-2 Cells
Transport of IRW, an Ovotransferrin-Derived Antihypertensive Peptide, in Human Intestinal Epithelial Caco-2 Cells
复制标题
DOI：
10.1021/jf302904t
复制
发表时间：
2013-02-20
影响因子：
6.1
通讯作者：
Wu, Jianping
中科院分区：
文献类型：
--
作者：
Bejjani, Satyanarayana;Wu, Jianping
IRW是卵转铁蛋白衍生的ACE抑制肽。本研究的目的是评价IRW在Caco-2细胞单层中的稳定性和跨细胞转运。在顶端（AP）表面监测IRW的稳定性，同时研究其从AP到基底（BL）和从BL到AP表面的转运。结果表明，IRW对肠肽酶耐药长达60分钟。IRW的运输不受添加胞吞转运抑制剂渥马菌素的影响。然而，在细胞松弛素D，间隙连接破坏剂的存在下，IRW的运输显着增加，这表明可能是被动运输从AP到BL表面。IRW从AP到BL表面的转运高于从BL到AP表面的转运，表明IRW是被动介导的转运。此外，在甘氨酰-肌氨酸的存在下，肽转运蛋白PepT 1的底物，IRW从AP到BL表面的转运减少。上述观察结果表明IRW在Caco-2细胞单层中的非典型转运。因此，IRW可能被完整地吸收到控制高血压的作用部位。
IRW is an egg ovotransferrin-derived ACE inhibitory peptide. The purpose of this study was to evaluate the stability and transcellular transport of IRW in Caco-2 cell monolayers. The stability of IRW was monitored on the apical (AP) surface while its transport was studied from AP to basal (BL) and from BL to AP surfaces. The results revealed that IRW is resistant against intestinal peptidase up to 60 min. Transport of IRW was not affected by addition of wortamanin, a transcytosis inhibitor. However, in the presence of cytochalasin D, a gap junction disruptor, transport of IRW was significantly increased, suggesting a possible passive transport from AP to BL surface. A higher transport of IRW from AP to BL surface than that from BL to AP surface suggests a passive-mediated transport. Moreover, in the presence of glycyl-sarcosine, a substrate for peptide transporter PepT 1, transport of IRW was reduced from AP to BL surface. The above observations showed atypical transport of IRW in Caco-2 cell monolayers. Thus, IRW may possibly be absorbed intact into the site of action for controlling hypertension.