Orientation of cohesive end site cos determines the active orientation of chi sequence in stimulating recA . recBC-mediated recombination in phage lambda lytic infections.
Orientation of cohesive end site cos determines the active orientation of chi sequence in stimulating recA . recBC-mediated recombination in phage lambda lytic infections.
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粘性末端位点cos的方向决定了chi序列在刺激recA时的活性方向。
DOI：
10.1073/pnas.79.19.5981
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发表时间：
1982
影响因子：
11.1
通讯作者：
F. Stahl
中科院分区：
文献类型：
--
作者：
I. Kobayashi;H. Murialdo;J. Crasemann;M. Stahl;F. Stahl
序列chi，5'G-C-T-G-G-T-G-G，局部增强大肠杆菌的recA和recBC蛋白的同源重组。先前的研究表明，在 lambda 噬菌体中，chi 在一个方向（向左）比另一个方向（向右）更活跃。反转 cos（lambda 成熟 DNA 末端的序列）可逆转这种方向依赖性：右侧的 chi 变得完全活跃，而左侧的 chi 变得相对不活跃。我们推测 lambda 噬菌体中的 chi 作用与 DNA 包装或注射相结合。
Sequence chi, 5'G-C-T-G-G-T-G-G, locally enhances homologous recombination by recA and recBC proteins of Escherichia coli. Previous work showed that, in phage lambda, chi is more active in one orientation (leftward) than in the other (rightward). Inverting cos, the sequence for the mature DNA ends of lambda, reverses this orientation dependence: the rightward chi becomes fully active, and the leftward chi becomes relatively inactive. We surmise that chi action in phage lambda is coupled with DNA packaging or injection.