Immunity regulatory DNAs share common organizational features in Drosophila
Immunity regulatory DNAs share common organizational features in Drosophila
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DOI：
10.1016/s1097-2765(03)00500-8
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发表时间：
2004-01-16
期刊：
影响因子：
16
通讯作者：
Levine, M
中科院分区：
文献类型：
--
作者：
Senger, K;Armstrong, GW;Levine, M
感染导致果蝇脂肪体中免疫基因的快速激活。两类转录因子参与了这一过程：含有REL的蛋白、背部蛋白、Dif蛋白和味觉蛋白，以及GATA因子蛇。在这里，我们提出了REL-GATA协同作用在免疫反应中发挥普遍作用的证据。SELEX分析确定了一致的结合位点，允许表征几个免疫调节DNA。这些DNA中REL和GATA位点的分布表明，大多数或所有脂肪特异性免疫基因都包含一个共同的调控元件组织：紧密相连的REL和GATA结合位点位于同一方向，位于转录起始点附近。这一“调节代码”的某些方面对于免疫反应是必不可少的。这些结果表明，免疫调节DNA含有受限的组织特征，这可能是真核细胞增强剂的一般特性。
Infection results in the rapid activation of immunity genes in the Drosophila fat body. Two classes of transcription factors have been implicated in this process: the REL-containing proteins, Dorsal, Dif, and Relish, and the GATA factor Serpent. Here we present evidence that REL-GATA synergy plays a pervasive role in the immune response. SELEX assays identified consensus binding sites that permitted the characterization of several immunity regulatory DNAs. The distribution of REL and GATA sites within these DNAs suggests that most or all fat-specific immunity genes contain a common organization of regulatory elements: closely linked REL and GATA binding sites positioned in the same orientation and located near the transcription start site. Aspects of this "regulatory code" are essential for the immune response. These results suggest that immunity regulatory DNAs contain constrained organizational features, which may be a general property of eukaryotic enhancers.