RhoGTPase-binding proteins, the exocyst complex and polarized vesicle trafficking.
RhoGTPase-binding proteins, the exocyst complex and polarized vesicle trafficking.
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DOI：
10.4161/sgtp.28453
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发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Aguilar, R Claudio
中科院分区：
文献类型：
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作者：
Mukherjee, Debarati;Sen, Arpita;Aguilar, R Claudio
细胞极性，即蛋白质和脂质的不对称分布，对各种细胞功能至关重要。协调细胞极性的一种机制是极化囊泡运输;由此负载货物的分泌囊泡被特异性地运输到细胞的预定区域。进化上保守的外囊复合物及其小的GT3调节剂在极化囊泡运输的时空控制中起着至关重要的作用。在神经元膜重塑和突触可塑性的研究中，保守的外囊调节机制和极化囊泡运输过程中的货物回收也开始出现。最近，我们的实验室证明，RhoGTP酶结合蛋白在酵母（Bem 3）和哺乳动物（Ocrl 1）也需要有效的交通的分泌囊泡的极化生长和信号的网站。与我们的研究一起，我们强调了在不同的细胞功能和模型系统中极化囊泡交通所必需的基本元素的进化保守性。总之，我们强调，RhoGTP酶结合蛋白在这些过程中的研究应包括在下一个层次的调查，更全面地了解他们迄今为止未知的作用，在极化膜交通和外囊调节。
Cell polarity, the asymmetric distribution of proteins and lipids, is essential for a variety of cellular functions. One mechanism orchestrating cell polarity is polarized vesicle trafficking; whereby cargo loaded secretory vesicles are specifically transported to predetermined areas of the cell. The evolutionarily conserved exocyst complex and its small GTPase regulators play crucial roles in spatiotemporal control of polarized vesicle trafficking. In studies on neuronal membrane remodeling and synaptic plasticity, conserved mechanisms of exocyst regulation and cargo recycling during polarized vesicle trafficking are beginning to emerge as well. Recently, our lab demonstrated that RhoGTPase-binding proteins in both yeast (Bem3) and mammals (Ocrl1) are also required for the efficient traffic of secretory vesicles to sites of polarized growth and signaling. Together with our studies, we highlight the evolutionary conservation of the basic elements essential for polarized vesicle traffic across different cellular functions and model systems. In conclusion, we emphasize that studies on RhoGTPase-binding proteins in these processes should be included in the next level of investigation, for a more complete understanding of their hitherto unknown roles in polarized membrane traffic and exocyst regulation.