Melting proteins confined in nanodroplets with 10.6 μm light provides clues about early steps of denaturation
Melting proteins confined in nanodroplets with 10.6 μm light provides clues about early steps of denaturation
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DOI：
10.1039/c7cc09829d
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发表时间：
2018-04-04
影响因子：
4.9
通讯作者：
Clemmer, David E.
中科院分区：
文献类型：
--
作者：
El-Baba, Tarick J.;Fuller, Daniel R.;Clemmer, David E.
用可变功率CO2激光照射纳米液滴内的泛素。质谱分析显示，在溶剂蒸发和离子形成之前，液滴内存在蛋白质“融化”样转变的证据。离子迁移谱表明，与早期步骤的变性相关联的结构被捕获，因为液滴寿命短。
Ubiquitin confined within nanodroplets was irradiated with a variablepower CO2 laser. Mass spectrometry analysis shows evidence for a protein "melting''-like transition within droplets prior to solvent evaporation and ion formation. Ion mobility spectrometry reveals that structures associated with early steps of denaturation are trapped because of short droplet lifetimes.