The sugar transporter SWEET10 acts downstream of FLOWERING LOCUS T during floral transition of Arabidopsis thaliana
The sugar transporter SWEET10 acts downstream of FLOWERING LOCUS T during floral transition of Arabidopsis thaliana
复制标题
DOI：
10.1186/s12870-020-2266-0
复制
发表时间：
2020-02-03
影响因子：
5.3
通讯作者：
Coupland, George
中科院分区：
文献类型：
--
作者：
Andres, Fernando;Kinoshita, Atsuko;Coupland, George
背景花转变是植物生殖发育的起始阶段，是对环境和内源信号的响应。在拟南芥中，这一过程被几种环境因素加速，包括暴露在长日照下。开花的光周期依赖性促进涉及叶的韧皮部中的FLOWLEDLOCUS T（FT）的转录诱导。FT编码一种移动的蛋白，该蛋白从叶片运输到茎顶端分生组织，在那里它形成诱导开花的调节复合物的一部分。FT是否在野生型植物的叶子中也具有生物学功能尚不清楚。结果为了解决这一问题，我们首先研究了与韧皮部伴随细胞中FT过表达相关的叶转录组变化。我们发现FT诱导SWEET 10的转录，SWEET 10编码双向蔗糖转运蛋白，特别是在叶脉中。此外，SWEET 10被长光周期转录激活，这种激活依赖于FT及其最早的靶基因之一恒定过度表达的供应者1（SOC 1）。SWEET 10的异位表达导致了植物的早花，并导致了茎尖中开花时间相关基因的高水平转录。总的来说，我们的研究结果表明，FT信号通路激活转录的蔗糖吸收/流出载体在花的过渡，表明它改变了开花植物的代谢，以及重新编程转录的花调节剂在芽分生组织。
Background Floral transition initiates reproductive development of plants and occurs in response to environmental and endogenous signals. In Arabidopsis thaliana, this process is accelerated by several environmental cues, including exposure to long days. The photoperiod-dependent promotion of flowering involves the transcriptional induction of FLOWERING LOCUS T (FT) in the phloem of the leaf. FT encodes a mobile protein that is transported from the leaves to the shoot apical meristem, where it forms part of a regulatory complex that induces flowering. Whether FT also has biological functions in leaves of wild-type plants remains unclear. Results In order to address this issue, we first studied the leaf transcriptomic changes associated with FT overexpression in the companion cells of the phloem. We found that FT induces the transcription of SWEET10, which encodes a bidirectional sucrose transporter, specifically in the leaf veins. Moreover, SWEET10 is transcriptionally activated by long photoperiods, and this activation depends on FT and one of its earliest target genes SUPPRESSOR OF CONSTANS OVEREXPRESSION 1 (SOC1). The ectopic expression of SWEET10 causes early flowering and leads to higher levels of transcription of flowering-time related genes in the shoot apex. Conclusions Collectively, our results suggest that the FT-signaling pathway activates the transcription of a sucrose uptake/efflux carrier during floral transition, indicating that it alters the metabolism of flowering plants as well as reprogramming the transcription of floral regulators in the shoot meristem.