Effect of Exosomes from Rat Adipose-Derived Mesenchymal Stem Cells on Neurite Outgrowth and Sciatic Nerve Regeneration After Crush Injury.
Effect of Exosomes from Rat Adipose-Derived Mesenchymal Stem Cells on Neurite Outgrowth and Sciatic Nerve Regeneration After Crush Injury.
复制标题
大鼠脂肪衍生的间充质干细胞的外泌体对挤压损伤后神经突生长和坐骨神经再生的影响。
DOI：
10.1007/s12035-018-1172-z
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发表时间：
2019-03
影响因子：
5.1
通讯作者：
Radtke C
中科院分区：
文献类型：
--
作者：
Bucan V;Vaslaitis D;Peck CT;Strauß S;Vogt PM;Radtke C
周围神经损伤后，无论是宏观环境还是微观环境都需要最佳条件来促进轴突再生。然而，大多数创伤性周围神经损伤的修复策略往往导致临床结果不令人满意。虽然已经采取了各种策略来改善宏观环境，但神经导管提供的微环境中轴突再生的潜在分子机制仍不清楚。在这项研究中，我们评估了脂肪间充质干细胞（adMSCs）来源的外泌体对坐骨神经再生和神经突起生长的影响。分子和免疫组织化学技术被用来研究特征性外泌体标志物的存在。建立了共培养体系，以确定外泌体对体外神经突起延伸的影响。通过足迹分析法评价大鼠在体行走行为，通过免疫细胞化学法评价神经再生。adMSC分泌称为外泌体的纳米囊泡，其在体外增加神经突生长并在体内增强坐骨神经损伤后的再生。此外，我们首次在adMSC外泌体中发现了神经生长因子转录本的存在。我们的研究结果表明，外泌体，组成性产生的adMSCs，参与周围神经再生，并有可能被用作有效的组织工程神经的治疗工具。
Peripheral nerve injury requires optimal conditions in both macro-environment and microenvironment for promotion of axonal regeneration. However, most repair strategies of traumatic peripheral nerve injury often lead to dissatisfying results in clinical outcome. Though various strategies have been carried out to improve the macro-environment, the underlying molecular mechanism of axon regeneration in the microenvironment provided by nerve conduit remains unclear. In this study, we evaluate the effects of from adipose-derived mesenchymal stem cells (adMSCs) originating exosomes with respect to sciatic nerve regeneration and neurite growth. Molecular and immunohistochemical techniques were used to investigate the presence of characteristic exosome markers. A co-culture system was established to determine the effect of exosomes on neurite elongation in vitro. The in vivo walking behaviour of rats was evaluated by footprint analysis, and the nerve regeneration was assessed by immunocytochemistry. adMSCs secrete nano-vesicles known as exosomes, which increase neurite outgrowth in vitro and enhance regeneration after sciatic nerve injury in vivo. Furthermore, we showed the presence of neural growth factors transcripts in adMSC exosomes for the first time. Our results demonstrate that exosomes, constitutively produced by adMSCs, are involved in peripheral nerve regeneration and have the potential to be utilised as a therapeutic tool for effective tissue-engineered nerves.
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影响因子：
3.7
作者：
Boomsma RA;Geenen DL
通讯作者：
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