Adsorption of histones on natural polysaccharides: The potential as agent for multiple organ failure in sepsis
Adsorption of histones on natural polysaccharides: The potential as agent for multiple organ failure in sepsis
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DOI：
10.1016/j.ijbiomac.2015.11.029
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发表时间：
2016-03-01
影响因子：
8.2
通讯作者：
Murata, Yoshifumi
中科院分区：
文献类型：
--
作者：
Isobe, Takashi;Kofuji, Kyoko;Murata, Yoshifumi
组蛋白是细胞内的蛋白质，是核染色质的结构元件并调节基因转录。然而，响应细菌挑战而释放的细胞外组蛋白已被鉴定为促成内皮功能障碍、器官衰竭和脓毒症期间死亡的介质。在本研究中，研究了组蛋白以及血浆蛋白（α（1）-酸性糖蛋白（AGP），白蛋白和γ-球蛋白）对海藻酸，果胶，葡聚糖和壳聚糖的吸附，以评估天然多糖作为脓毒症多器官功能衰竭治疗药物的潜力。海藻酸和果胶对组蛋白有较强的吸附作用，而葡聚糖和壳聚糖对组蛋白的吸附能力很低或可以忽略不计。在检查的天然多糖中，只有海藻酸不吸附任何血浆蛋白。这些结果表明，海藻酸强烈吸附组蛋白，但不是血浆蛋白，因此，它有潜力作为一个候选药物治疗多器官衰竭的脓毒症。(C)2015 Elsevier B. V.版权所有。
Histones are intracellular proteins that are structural elements of nuclear chromatin and regulate gene transcription. However, the extracellular histones released in response to bacterial challenges have been identified as mediators contributing to endothelial dysfunction, organ failure, and death during sepsis. In the present study, the adsorption of histones as well as plasma proteins (alpha(1)-acid glycoprotein (AGP), albumin, and gamma-globulin) on alginic acid, pectin, dextran, and chitosan was examined in order to evaluate the potential of natural polysaccharides as therapeutic agents for multiple organ failure in sepsis. Alginic acid and pectin strongly adsorbed histones, whereas the adsorption abilities of dextran and chitosan toward histones were very low or negligible. Among the natural polysaccharides examined, only alginic acid did not adsorb any of the plasma proteins. These results demonstrated that alginic acid strongly adsorbed histones, but not plasma proteins; therefore, it has potential as a candidate drug for the treatment of multiple organ failure in sepsis. (C) 2015 Elsevier B.V. All rights reserved.