Neurospheres from human adipose tissue transplanted into cultured mouse embryos can contribute to craniofacial morphogenesis: A preliminary report
Neurospheres from human adipose tissue transplanted into cultured mouse embryos can contribute to craniofacial morphogenesis: A preliminary report
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DOI：
10.1097/01.scs.0000249357.66720.d2
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发表时间：
2007-01-01
影响因子：
0.9
通讯作者：
Machida, Masafumi
中科院分区：
文献类型：
--
作者：
Nagase, Takashi;Matsumoto, Daisuke;Machida, Masafumi
脂肪源性基质细胞（Adipose-derived stromal cells，ASCs）是最有前途的干细胞群之一，在体外可分化为中胚层和神经谱系。在这项研究中，我们研究了神经球培养方法的人ASCs的神经分化潜力。来源于人ASCs的神经球表达Nestin和Musashi-1基因，这是神经干细胞的标记基因。当这些细胞用仙台病毒载体转染的绿色荧光蛋白基因标记并使用全胚胎培养系统移植到小鼠胚胎的头部区域时，这些细胞被并入颅面发育。一些移植的细胞似乎沿着第二鳃弓迁移，这表明与颅神经嵴细胞有一些相似之处。尽管是初步的，但我们的结果支持了ASC衍生的神经球在体外和体内具有神经祖细胞特性的观点。
Adipose-derived stromal cells (ASCs) are one of the most promising stem cell populations that differentiate into the mesodermal as well as neural lineages in vitro. In this study, we examined the neural differentiating potential of human ASCs by a neurosphere culture method. Neurospheres derived from human ASCs expressed Nestin and Musashi-1 genes, which are marker genes for neural stem cells. When these cells were labeled with green fluorescent protein gene transfection by Sendai virus vector and transplanted into the head region of mouse embryos using a whole embryo culture system, these cells were incorporated into the craniofacial development. Some transplanted cells appeared to migrate along the second branchial arches, implicating some similarity to the cranial neural crest cells. Although preliminary, our results support an idea that ASC-derived neurospheres have properties of neural progenitors in vitro and in vivo.