High resolution scanning electron microscopy of the cell
High resolution scanning electron microscopy of the cell
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DOI：
10.1111/j.1768-322x.1989.tb00777.x
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发表时间：
1989
影响因子：
2.7
通讯作者：
Kéiichi Tanaka
中科院分区：
文献类型：
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作者：
Kéiichi Tanaka
随着扫描电子显微镜分辨率的提高和样品制备技术的进步，扫描电子显微镜已成为研究超微结构的有力工具。在分辨率方面，在这十年中逐步提高，并于1985年开发出超高分辨率SEM（UHS-T1），分辨率为0.5 nm。关于样品制备，锇-DMSO-锇法，这是有效地揭示细胞内结构，已被广泛使用。近年来，人们还发明了观察较小物体的技术，如噬菌体、病毒和生物大分子。由于这些制备技术和超高分辨率SEM的可用性，SEM在生物学中的应用正在迅速扩大。在本文中，超高分辨率扫描电镜的概述，标本制备技术，通过这些技术的一些生物材料的发现，以及制作标本的指导方针，进行了描述。
The scanning electron microscope (SEM) has become a powerful tool for ultrastructural research with improvement of the instrument's resolution and progress in specimen preparation techniques. With regard to resolution, it has been improved step‐by‐step in this decade and, in 1985, an ultra‐high resolution SEM (UHS‐T1) was developed, with a resolution of 0.5 nm. Concerning specimen preparation, the osmium‐DMSO‐osmium method, which is effective for revealing intracellular structures, has come to be widely used. Techniques for observing smaller objects, such as bacteriophages, viruses, and biological macromolecules, have also been devised in recent years. As a result of these preparation techniques and the availability of the ultra‐high resolution SEM, the application of SEM in biology is expanding rapidly. In this paper, an outline of the ultra‐high resolution SEM, techniques for specimen preparation, findings of some biological materials by these techniques, and guidelines to making the specimens, are described.