Brain biopsy in Creutzfeldt–Jakob disease: evolution of pathological changes by prion protein immunohistochemistry
Brain biopsy in Creutzfeldt–Jakob disease: evolution of pathological changes by prion protein immunohistochemistry
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克雅氏病的脑活检：朊病毒蛋白免疫组织化学病理变化的演变
DOI：
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发表时间：
2002
影响因子：
5
通讯作者：
P. Satishchandra
中科院分区：
文献类型：
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作者：
A. Mahadevan;S. Shankar;T. Yasha;V. Santosh;C. Sarkar;A. Desai;P. Satishchandra
蛋白酶抗性朊病毒蛋白（PrPsc）的形成被认为是克雅氏病（CJD）发病机制的早期事件，因此其在脑活检中的免疫组织化学表现被认为是诊断性的。我们分析了8例脑活检从额叶皮层收集来自印度不同地区的病例诊断为CJD的临床和病理的朊病毒蛋白（PrP）的表达。这些病例的病程从2个月到1年不等。使用两种不同的PrP单克隆抗体克隆（KG 9和3F 4）在石蜡切片上进行免疫组织化学。尽管所有8例病例均表现出不同严重程度的海绵状脑病的典型特征，但8例病例中仅5例显示脑组织中存在PrPsc。免疫标记是局灶性的，所有具有海绵状变化的区域均未标记。染色模式的时间演变是明显的-早期阶段（2个月）的颗粒弥散标记（突触型），中期阶段（5-6个月）的空泡周围沉积物和晚期阶段（12个月）的致密斑块。
The formation of protease‐resistant prion protein (PrPsc) is considered to be an early event in the pathogenesis of Creutzfeldt–Jakob disease (CJD) and hence its demonstration in brain biopsies by immunohistochemistry is considered diagnostic. We analysed eight brain biopsies from the frontal cortex collected from different parts of India from cases diagnosed as CJD on clinical and pathological grounds for the expression of prion protein (PrP). The duration of illness in these cases varied from 2 months to 1 year. Immunohistochemistry was carried out on paraffin sections using two different clones (KG9 and 3F4) of monoclonal antibodies to PrP. Although all eight cases showed classical features of spongiform encephalopathy of varying severity, only five of the eight cases revealed PrPsc in the brain tissue. The immunolabelling was focal and all areas with spongiform change were not labelled. A temporal evolution in the staining pattern was evident – particulate diffuse labelling (synaptic type) in early stages (2 months), perivacuolar deposits in intermediate stages (5–6 months), and dense plaques in late stages (12 months).
朊病毒疾病的病因学和发病机制。
DOI：
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发表时间：
1995
期刊：
The American journal of pathology.
影响因子：
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作者：
DeArmond,SJ;Prusiner,SB
通讯作者：
Prusiner,SB