A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
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DOI：
10.1126/science.1062961
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发表时间：
2001-08-03
期刊：
影响因子：
56.9
通讯作者：
Zamore, PD
中科院分区：
文献类型：
--
作者：
Hutvágner, G;McLachlan, J;Zamore, PD
21个核苷酸的小时间RNA（stRNA）let-7调节秀丽隐杆线虫的发育时间，并可能在其他双边动物。我们目前在体内和体外的证据表明，在果蝇发育调节前体RNA被切割的RNA干扰样机制，以产生成熟的let-7的stRNA。在培养的人类细胞中，靶向破坏编码酶Dicer的信使RNA，其在RNA干扰途径中起作用，导致let-7前体的积累。因此，RNA干扰和stRNA途径交叉。这两种途径都需要RNA加工酶Dicer来产生抑制基因表达的活性小RNA成分。
The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.