Identification of functional cis-elements required for repression of the Taka-amylase A gene under secretion stress in Aspergillus oryzae
Identification of functional cis-elements required for repression of the Taka-amylase A gene under secretion stress in Aspergillus oryzae
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米曲霉分泌应激下抑制 Taka-淀粉酶 A 基因所需的功能顺式元件的鉴定
DOI：
10.1007/s10529-014-1691-2
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发表时间：
2015-02
影响因子：
2.7
通讯作者：
Pan, L.
中科院分区：
文献类型：
--
作者：
Zhou, Bin;Wang, Chao;Wang, Bin;Li, Xiupeng;Xiao, Jing;Pan, L.
丝状真菌在内质网胁迫下，分泌蛋白的表达受到转录反馈机制的下调，称为分泌胁迫下的抑制(RESS)。为了探讨RESS的机制，我们分析了分泌型蛋白胁迫抑制的Taka-amylase A基因(MyB)在米曲霉中的表达。我们对myB启动子进行了截短和缺失分析，以确定RESS所需的顺式元件。RESS需要与参与淀粉分解基因表达的转录激活剂−R没有任何结合位点的核苷酸序列(位置−378至AMY291)。八聚体序列TCACGGGC(位置−307至−300)构成了分泌胁迫下下调myB所必需的上游激活元件的核心序列。内质网应激下，AmyR和RESS的失活均导致了AmyB表达的下调。
The expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (RESS). To investigate the RESS mechanism, we analyzed the expression of the Taka-amylase A gene (amyB) in Aspergillus oryzae, which was depressed under secreted protein stress. We conducted a truncation and deletion analysis of the amyB promoter to identify cis-elements required for RESS. A nucleotide sequence (positions −378 to −291) without any binding sites for the transcriptional activator AmyR, which is involved in amylolytic gene expression, was required for RESS. The octamer sequence TCACGGGC (positions −307 to −300) constituted the core sequence of the upstream activating element essential for amyB down-regulation under secretion stress. Both the inactivation of AmyR and RESS contributed to the down-regulation of amyB expression under ER stress.
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影响因子：
4.4
作者：
Carvalho ND;Jørgensen TR;Arentshorst M;Nitsche BM;van den Hondel CA;Archer DB;Ram AF
通讯作者：
Ram AF
影响因子：
4.4
作者：
Andrew H. Sims;M. E. Gent;K. Lanthaler;N. Dunn-Coleman;S. Oliver;G. Robson
通讯作者：
Andrew H. Sims;M. E. Gent;K. Lanthaler;N. Dunn-Coleman;S. Oliver;G. Robson
影响因子：
4.4
作者：
Nakajima, Ken-ichiro;Asakura, Tomiko;Abe, Keiko
通讯作者：
Abe, Keiko
影响因子：
3.6
作者：
Al-Sheikh, H;Watson, AJ;Archer, DB
通讯作者：
Archer, DB
影响因子：
4.1
作者：
Lee, Changsoo;Kim, Jaai;Hwang, Seokhwan
通讯作者：
Hwang, Seokhwan