Increase of SUMO-1 expression in response to hypoxia:: direct interaction with HIF-1α in adult mouse brain and heart in vivo
Increase of SUMO-1 expression in response to hypoxia:: direct interaction with HIF-1α in adult mouse brain and heart in vivo
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DOI：
10.1016/j.febslet.2004.05.079
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发表时间：
2004-07-02
期刊：
影响因子：
3.5
通讯作者：
Billig, H
中科院分区：
文献类型：
--
作者：
Shao, RJ;Zhang, FP;Billig, H
本研究探讨了小泛素相关修饰物-1（SUMO-1）在成年小鼠脑和心脏缺氧反应中的表达调控。我们观察到SUMO-1 mRNA和蛋白在体内缺氧刺激后显著增加。由于SUMO-1与多种转录因子相互作用，包括缺氧诱导因子-1 β（HIF-1 β）在体外，我们不仅证明了HIF-1 α的表达在大脑和心脏缺氧增加，但也提供了证据，表明SUMO-1与HIF-1 α在体内共定位响应缺氧，通过证明这两种蛋白在神经元和心肌细胞的共表达。SUMO-1和HIF-1 α之间的特异性相互作用也通过免疫共沉淀得到证实。这些结果表明，SUMO-1水平的增加通过SUMO化参与脑和心脏中HIF-1 α功能的调节。(C)2004年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
The present study investigates the regulation of small ubiquitin-related modifier-1 (SUMO-1) expression in response to hypoxia in adult mouse brain and heart. We observed a significant increase in SUMO-1 mRNAs and proteins after hypoxic stimulation in vivo. Because SUMO-I interacts with various transcription factors, including hypoxia-inducible factor-1beta (HIF-1beta) in vitro, we not only demonstrated that the HIF-1alpha expression is increased by hypoxia in brain and heart, but also provided evidence that SUMO-1 co-localizes in vivo with HIF-1alpha in response to hypoxia by demonstrating the co-expression of these two proteins in neurons and cardiomyocytes. The specific interaction between SUMO-1 and HIF-1alpha was additionally demonstrated with co-immunoprecipitation. These results indicate that the increased levels of SUMO-1 participate in the modulation of HIF-1alpha function through sumoylation in brain and heart. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.