Antifungal activity of the essential oil of Angelica major against Candida, Cryptococcus, Aspergillus and dermatophyte species
Antifungal activity of the essential oil of Angelica major against Candida, Cryptococcus, Aspergillus and dermatophyte species
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DOI：
10.1007/s11418-014-0884-2
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发表时间：
2015-04-01
影响因子：
3.3
通讯作者：
Pinto, Eugenia
中科院分区：
文献类型：
--
作者：
Cavaleiro, Carlos;Salgueiro, Ligia;Pinto, Eugenia
研究了大当归挥发油及其主要成分α-蒎烯和顺式-β-罗勒烯的组成及其对临床相关酵母菌和霉菌的抗真菌活性。从植物的地上部分的EO是通过水蒸馏和气相色谱（GC）和气相色谱/质谱（GC/MS）分析。该油显示出高含量的α-蒎烯（21.8%）和顺式-β-罗勒烯（30.4%）。根据临床和实验室标准化研究所（CLSI）的肉汤大量稀释方案测量最小抑菌浓度（MIC）。EO、α-蒎烯和顺式-β-罗勒烯对皮肤癣菌和新型隐球菌的MIC和最小杀菌浓度（MFC）较低，其中α-蒎烯活性最高。关于念珠菌属，EO敏感性特征似乎是多样的，与氟康唑敏感性模式无关。此外，在亚抑制浓度的EO、α-蒎烯和顺式-β-罗勒烯下，C.白色念珠菌此外，它们的溶血活性低。由A.主要EO及其主要成分与低细胞毒性活性相关，证实了它们作为抗真菌剂的潜力，可用于对抗经常与人类真菌病有关的真菌，特别是隐球菌病和皮肤真菌病。与商业抗真菌化合物的结合可以通过对芽管形成的影响带来益处，并且可以用于皮肤粘膜念珠菌病的治疗。
The composition and antifungal activity of the essential oil (EO) of Angelica major and its main components alpha-pinene and cis-beta-ocimene against clinically relevant yeasts and moulds were evaluated. EO from the plant's aerial parts was obtained by hydrodistillation and analysed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The oil showed high contents of alpha-pinene (21.8 %) and cis-beta-ocimene (30.4 %). Minimum inhibitory concentrations (MICs) were measured according to the broth macrodilution protocols by the Clinical and Laboratory Standards Institute (CLSI). The EO, alpha-pinene and cis-beta-ocimene displayed low MICs and minimum fungicidal concentrations (MFCs) against dermatophytes and Cryptococcus neoformans, with alpha-pinene being the most active. Regarding Candida species, the EO susceptibility profiles seem to be diverse and not correlated with fluconazole susceptibility patterns. Moreover, an inhibition of yeast-mycelium transition was demonstrated at sub-inhibitory concentrations of the EO, alpha-pinene and cis-beta-ocimene in C. albicans. In addition, their haemolytic activity was low. The activity displayed by A. major EO and its main components associated with low cytotoxic activity confirms their potential as an antifungal agent against fungal species frequently implicated in human mycoses, particularly cryptococcosis and dermatophytosis. The association with commercial antifungal compounds could bring benefits, by the effect on germ tube formation, and be used in mucocutaneous candidiasis treatment.