NUMBER AND DISTRIBUTION OF POLYADENYLATED RNA SEQUENCES IN YEAST
NUMBER AND DISTRIBUTION OF POLYADENYLATED RNA SEQUENCES IN YEAST
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DOI：
10.1016/0092-8674(77)90032-0
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发表时间：
1977-01-01
期刊：
影响因子：
64.5
通讯作者：
ROSBASH, M
中科院分区：
文献类型：
--
作者：
HEREFORD, LM;ROSBASH, M
从芽殖酵母酿酒酵母中分离出的含有poly(A)的RNA，通过RNA-c[互补]DNA杂交的动力学分析来表征序列的数量和分布。与先前在后生动物真核生物中获得的结果一致，观察到了离散的复杂性类别。存在低、中、高复杂度类别，分别包含大约 20、400 和 2400 个序列。通过与单拷贝 DNA 杂交来验证序列数量的测量。酵母基因组中 20% 的单拷贝部分由含有 Poly(A) 的 RNA 形成双链。假设不对称转录，这相当于大约 4000 个含有 Poly(A) 的序列，验证了 RNA-cDNA 杂交获得的结果。与单拷贝 DNA 杂交的一级动力学验证了大多数序列复杂性存在于相同细胞内浓度的概念。在含有 Poly(A) 的多核糖体 RNA 和总 RNA 中发现了相同数量和分布的序列，表明大部分或全部序列复杂性位于多核糖体上并且被腺苷酸化。显然，RNA-cDNA 杂交是确定序列复杂性值的准确方法，在营养条件下生长的酵母具有 3000-4000 个不同的 mRNA 序列。
The poly(A)-containing RNA, isolated from the budding yeast Saccharomyces cerevisiae, was characterized with regard to the number and distribution of sequences by a kinetic analysis of RNA-c[complementary]DNA hybridization. In agreement with results previously obtained on metazoan eukaryotes discrete complexity classes were observed. There exist low, medium and high complexity classes which contain approximately 20, 400 and 2400 sequences, respectively. This measurement of the number of sequences was verified by hybridization with single copy DNA. Twenty percent of the single copy fraction of the yeast genome is rendered double-stranded by poly(A)-containing RNA. Assuming asymmetric transcription, this is equivalent to approximately 4000 poly(A)-containing sequences, verifying the results obtained with RNA-cDNA hybridization. The 1st-order kinetics of the hybridization with single copy DNA verified the notion that most of the sequence complexity is present at the same intracellular concentration. The same number and distribution of sequences were found in poly(A)-containing polysomal RNA and in total RNA, suggesting that most or all of the sequence complexity is on polysomes and is adenylated. Apparently RNA-cDNA hybridization is an accurate method for determining sequence complexity values and yeast, grown under vegetative conditions, has 3000-4000 different mRNA sequences.