Cell fractionation studies indicate that dystrophin is a protein of surface membranes of skeletal muscle.
Cell fractionation studies indicate that dystrophin is a protein of surface membranes of skeletal muscle.
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细胞分级分离研究表明肌营养不良蛋白是骨骼肌表面膜的蛋白质。
DOI：
10.1042/bj2580837
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Dimauro,S
中科院分区：
文献类型：
--
作者：
Salviati,G;Betto,R;Ceoldo,S;Biasia,E;Bonilla,E;Miranda,AF;Dimauro,S
我们研究了肌营养不良蛋白在兔骨骼肌中的亚细胞定位。在蛋白质印迹分析的膜制剂，肌营养不良蛋白与肌膜部分，所示的胆固醇含量和共纯化哇巴因结合活性和β-肾上腺素能受体。肌营养不良蛋白也被发现与连接的T-小管，但不与“自由”的T-小管，纵向部分或终端池的肌浆网。抗肌营养不良蛋白不被高盐溶液溶解，但它被低浓度的洗涤剂（Triton X-100和脱氧胆酸盐）溶解，这表明它是一种外周膜蛋白。
We studied the subcellular localization of dystrophin in rabbit skeletal muscle. In Western-blot analysis of membrane preparations, dystrophin was associated with the sarcolemmal fraction, as indicated by cholesterol content and co-purification with ouabain-binding activity and beta-adrenergic receptor. Dystrophin was also found with junctional T-tubules, but not with ‘free’ T-tubules, longitudinal portions or terminal cisternae of the sarcoplasmic reticulum. Dystrophin was not solubilized by high salt solutions, but it was solubilized by low concentrations of detergents (Triton X-100 and deoxycholate), suggesting that it is a peripheral membrane protein.