Specificity of human, rat and mouse skin epoxide hydratase towards K-region epoxides of polycyclic hydrocarbons.
Specificity of human, rat and mouse skin epoxide hydratase towards K-region epoxides of polycyclic hydrocarbons.
复制标题
人、大鼠和小鼠皮肤环氧化物水合酶对多环烃 K 区环氧化物的特异性。
DOI：
10.1016/0006-2952(78)90251-4
复制
发表时间：
1978
影响因子：
5.8
通讯作者：
P. Bentley
中科院分区：
文献类型：
--
作者：
F. Oesch;H. Schmassmann;P. Bentley
已经在小鼠、大鼠和人类的皮肤微粒体部分中测量了环氧化物水合酶的活性。这种皮肤酶能够使所有测试的环氧化物水合。比活力按人、小鼠、大鼠的顺序递减。3种动物皮肤微粒体组分中各种多环烃对K区环氧化物的相对活性由大到小的顺序为：菲9，10-氧化物>；Benz(A)菲5，6-氧化物≃苯并(A)并(A)芘4，5-氧化物≃7-甲基苯并(A)菲5，6-氧化物>；3-甲基胆菲11，12-氧化物>；二苯并(a，h)菲5，6-氧化物。人皮肤微粒体组分中的环氧化物水合酶的活性几乎不受pH的影响，并可被小分子量抑制剂抑制，其抑制方式与肝微粒体酶相似。6名受试者皮肤微粒体环氧化物水合酶活力的个体间差异很大，即每毫克蛋白质的4，5-二氢二醇含量为175-447pmolo(A)Py。这种变异不是由于皮肤病或治疗引起的，也与年龄或性别没有明显的相关性。由于腹部皮肤样本的活性似乎低于腿部或胸部皮肤样本的活性，因此不能排除与采集皮肤样本的身体部位可能存在的相关性。
Epoxide hydratase activity has been measured in microsomal fractions of skin from mouse, rat and humans. The skin enzyme was able to hydrate all epoxides tested. The specific enzyme activities decreased in the order human > mouse > rat. The relative activity towards K-region epoxides of various polycyclic hydrocarbons in skin microsomal fractions from all three species decreased in the order phenanthrene 9,10-oxide > benz(a)anthracene 5,6-oxide ≃ benzo(a)pyrene 4,5-oxide ≃ 7-methylbenz(a)anthracene 5,6-oxide > 3-methylcholanthrene 11,12-oxide > dibenz(a,h)anthracene 5,6-oxide. The activity of epoxide hydratase in human skin microsomal fractions showed little pH dependence and was inhibited by small molecular weight inhibitors in a manner similar to that of the liver microsomal enzyme. Interindividual variation of epoxide hydratase activity in skin microsomal fractions from six human subjects was considerable, namely from 175 to 447 pmoles benzo(a)pyrene 4,5-dihydrodiol/min per mg protein. This variation was not due to skin disease or treatment and had no apparent correlation with age or sex. A possible correlation with the part of the body from which the skin sample was taken could not be excluded since the activity in skin samples from the abdomen seemed lower than that in samples from leg or breast.