A photoautotrophic platform for the sustainable production of valuable plant natural products from CO2
A photoautotrophic platform for the sustainable production of valuable plant natural products from CO2
复制标题
用于利用二氧化碳可持续生产有价值的植物天然产品的光合自养平台
DOI：
10.1039/c6gc00317f
复制
发表时间：
2016-01-01
期刊：
影响因子：
9.8
通讯作者：
Xu, Ping
中科院分区：
文献类型：
--
作者：
Ni, Jun;Tao, Fei;Xu, Ping
许多植物天然产物具有显着的药理活性。它们主要是从高等植物中直接提取生产的，很难满足全球不断增长的需求。此外，许多药用植物的过度砍伐对生态平衡产生不良影响。在这项研究中，我们利用单细胞蓝藻细长聚球藻PCC7942构建了一个光合自养平台，将温室气体二氧化碳直接转化为一系列有价值的保健产品，包括白藜芦醇、柚皮素、双去甲氧基姜黄素、对香豆酸、咖啡酸和阿魏酸。这六种化合物可以进一步分支成许多其他珍贵且有用的天然产物。已经系统地研究了各种策略来提高产量，包括引入反馈抑制抗性酶、创建功能性融合蛋白和增加丙二酰辅酶A供应。光合自养系统中这些天然产物的最高滴度达到 4.1–128.2 mg L−1，这与许多其他异养微生物利用碳水化合物获得的滴度高度可比。独立于碳水化合物原料、功能性组装 P450 以及丰富的 NADPH 和 ATP 的可用性等多项优势支持该光合作用平台特别适合生产植物天然产品。该平台还提供了一条将二氧化碳直接转化为许多芳香族结构单元的绿色途径，这是基于石化的散装芳香族化合物生产的一种有前途的替代方案。
Many plant natural products have remarkable pharmacological activities. They are mainly produced directly by extraction from higher plants, which can hardly keep up with the surging global demand. Furthermore, the over-felling of many medicinal plants has undesirable effects on the ecological balance. In this study, we constructed a photoautotrophic platform with the unicellular cyanobacterium Synechococcus elongatus PCC7942 to directly convert the greenhouse gas CO2 into an array of valuable healthcare products, including resveratrol, naringenin, bisdemethoxycurcumin, p-coumaric acid, caffeic acid, and ferulic acid. These six compounds can be further branched to many other precious and useful natural products. Various strategies including introducing a feedback-inhibition-resistant enzyme, creating functional fusion proteins, and increasing malonyl-CoA supply have been systematically investigated to increase the production. The highest titers of these natural products reached 4.1–128.2 mg L−1 from the photoautotrophic system, which are highly comparable with those obtained by many other heterotrophic microorganisms using carbohydrates. Several advantages such as independence from carbohydrate feedstocks, functionally assembling P450s, and availability of plentiful NADPH and ATP support that this photosynthetic platform is uniquely suited for producing plant natural products. This platform also provides a green route for direct conversion of CO2 to many aromatic building blocks, a promising alternative to petrochemical-based production of bulk aromatic compounds.