Influence of DNA delivery method on gene targeting frequencies in human cells
Influence of DNA delivery method on gene targeting frequencies in human cells
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DOI：
10.1023/b:scam.0000007137.28557.73
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发表时间：
1999-01-01
期刊：
影响因子：
--
通讯作者：
Porter, ACG
中科院分区：
文献类型：
--
作者：
Yáñez, RJ;Porter, ACG
Gene targeting can be used for genetic studies of human cell lines and has significant potential for somatic cell gene therapy. These applications are however restricted by the low frequency of homologous recombination in higher eukaryotes compared to the relatively efficient nonhomologous integration of transfected DNA into the genome. As part of our attempts to overcome this problem, we compared two widely used transfection methods for their efficiency in gene targeting. To our surprise we found that, for conditions that render similar frequencies of nonhomologous integrants, lipofection is much less efficient than electroporation in generating targeted clones. This suggests that nonhomologous and homologous recombination have different requirements for DNA delivery in human cells.
