OXYGEN RADICALS, LIPID-PEROXIDATION, AND PERMEABILITY CHANGES AFTER INTESTINAL ISCHEMIA AND REPERFUSION
OXYGEN RADICALS, LIPID-PEROXIDATION, AND PERMEABILITY CHANGES AFTER INTESTINAL ISCHEMIA AND REPERFUSION
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DOI：
10.1152/jappl.1993.74.4.1515
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发表时间：
1993-04-01
影响因子：
3.3
通讯作者：
WALKER, PB
中科院分区：
文献类型：
--
作者：
HORTON, JW;WALKER, PB
本研究检查了21-氨基类固醇U-74389对肠缺血[用无创夹阻断上级肠系膜动脉（SMA）和侧支血管20 min]期间和之后脂质过氧化[通过血浆和组织丙二醛（MDA）水平测定]、肠通透性（Cr-51标记EDTA的血浆-管腔清除率）和肠血流（激光多普勒）的影响。未经治疗的缺血增加EDTA清除率（从0.050 +/- 0.005至0.169 +/-0.005）。040毫升。min-1。100 g-1; n = 16，P = < 0.05），SMA流量减少88%（P < 0.05），血浆MDA增加（0.340 +/- 0.120至4.030 +/- 0.86 nmol/ml; n = 8，P = 0.01）;再灌注2 h进一步增加EDTA清除率（0.323 +/- 0.060 ml.mg-1.100 g-1）。在假缺血大鼠中，EDTA清除率在整个实验期间从基线保持不变（n = 12，0.060 +/- 0.006 ml . min-1。100 g-1）。缺血（n = 10）或再灌注（n = 11）时氨基类固醇治疗使EDTA清除率恢复到接近基线（基线0.071 +/-0.023;再灌注0.091 +/-0.014 ml）。min-1。100 g组织-1），并减少缺血-再灌注相关的组织MDA的升高。再灌注后2小时，所有实验组的SMA血流量均高于基线值。我们的数据表明，肠缺血和再灌注期间产生的氧源性自由基有助于1）细胞膜的脂质过氧化和2）肠粘膜通透性增加，增强细菌移位和脓毒症。
This study examined the effects of a 21-aminosteroid, U-74389, on lipid peroxidation [determined by plasma and tissue malondialdehyde (MDA) levels], intestinal permeability (plasma-to-luminal clearance of Cr-51-labeled EDTA), and intestinal blood flow (laser Doppler) during and after intestinal ischemia [superior mesenteric artery (SMA) and collateral vessel occlusion for 20 min with atraumatic clip]. Untreated ischemia increased EDTA clearance (from 0.050 +/- 0.005 to 0.169 +/- 0. 040 ml . min-1 . 100 g-1; n = 16, P = < 0.05), reduced SMA flow 88% (P < 0.05), and increased plasma MDA (0.340 +/- 0.120 to 4.030 +/- 0.86 nmol/ml; n = 8, P = 0.01); 2 h of reperfusion further increased EDTA clearance (0.323 +/- 0.060 ml.mg-1.100 g-1). EDTA clearance remained unchanged from baseline throughout the experimental period in sham ischemic rats (n = 12, 0.060 +/- 0.006 ml . min-1 . 100 g-1). Aminosteroid treatment at ischemia (n = 10) or with reperfusion (n = 11) returned EDTA clearance to near baseline (baseline 0.071 +/-0.023; reperfusion 0.091 +/- 0.014 ml . min-1 . 100 g tissue-1) and reduced the ischemia-reperfusion-associated rise in tissue MDA. Two hours after reperfusion, SMA blood flow was above baseline values in all experimental groups. Our data suggest that oxygen-derived free radicals produced during intestinal ischemia and reperfusion contribute to 1) lipid peroxidation of cell membranes and 2) increases in intestinal mucosal permeability, potentiating bacterial translocation and sepsis.