A genetically encoded photosensitizer
A genetically encoded photosensitizer
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DOI：
10.1038/nbt1175
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发表时间：
2006-01-01
影响因子：
46.9
通讯作者：
Lukyanov, KA
中科院分区：
文献类型：
--
作者：
Bulina, ME;Chudakov, DM;Lukyanov, KA
光敏剂是在光照射时产生活性氧（ROS）的发色团（1）。它们用于通过发色团辅助光灭活（卡利）灭活特定蛋白质，并用于光动力治疗中的光诱导细胞杀伤。在这里，我们报告了一种基因编码的光敏剂，我们称之为KillerRed，从水螅体色素蛋白anm 2CP，一个同系物的绿色荧光蛋白（GFP）。KillerRed在用绿色光照射时产生ROS。虽然已知的光敏剂必须外源地添加到生命系统中，但KillerRed是完全遗传编码的。我们证明了KillerRed用于光诱导的大肠杆菌和真核细胞的杀伤以及用于灭活与β-半乳糖苷酶和磷脂酶C δ 1普列克底物蛋白同源结构域的融合的效用。
Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation(1). They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced cell killing in photodynamic therapy. Here we report a genetically encoded photosensitizer, which we call KillerRed, developed from the hydrozoan chromoprotein anm2CP, a homolog of green fluorescent protein (GFP). KillerRed generates ROS upon irradiation with green light. Whereas known photosensitizers must be added to living systems exogenously, KillerRed is fully genetically encoded. We demonstrate the utility of KillerRed for light-induced killing of Escherichia coli and eukaryotic cells and for inactivating fusions to beta-galactosidase and phospholipase C delta 1 pleckstrin homology domain.