Mast cells distribution in human liver disease and experimental rat liver fibrosis. Indications for mast cell participation in development of liver fibrosis
Mast cells distribution in human liver disease and experimental rat liver fibrosis. Indications for mast cell participation in development of liver fibrosis
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DOI：
10.1016/s0168-8278(97)80113-4
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发表时间：
1997-05-01
影响因子：
25.7
通讯作者：
Ramadori, G
中科院分区：
文献类型：
--
作者：
Armbrust, T;Batusic, D;Ramadori, G
背景/目的：慢性肝病引起的肝纤维化的发展被认为是由炎症细胞释放激活脂肪储存细胞（ito细胞）的纤维化介质介导的。最近，肥大细胞参与了纤维的形成。我们研究了这些细胞在正常人类肝脏、非纤维化和纤维化肝脏疾病以及正常大鼠肝脏和急性和慢性损伤大鼠肝脏（CCl4模型）中的分布。方法：采用组织化学和免疫组织化学方法鉴定肥大细胞，比较大鼠肝脏和腹膜肥大细胞对蛇形蛋白α 1-抗胰蛋白酶、α 1-抗凝乳胰蛋白酶和抗凝血酶III的免疫反应性。结果：在正常人和大鼠肝脏中，门静脉中很少发现肥大细胞，非纤维化人和急性损伤的大鼠肝脏中细胞数量没有变化，相反，肝硬化人和大鼠肝脏门静脉和纤维间隔中含有大量肥大细胞，它们对蛇形蛋白表现出很强的免疫反应性，大鼠腹膜肥大细胞也是如此。结论：在肝纤维化晚期，肥大细胞可能在纤维化间隔处表现出蛋白酶抑制活性。
Background/Aims: The development of liver fibrosis due to chronic liver diseases is thought to be mediated by inflammatory cells releasing fibrogenic mediators that activate fat-storing cells (Ito-cells). Recently, the involvement of mast cells in fibrogenesis has been suggested. We studied the distribution of these cells in normal human liver and human nonfibrotic and fibrotic liver disease as web as in normal rat liver and acutely and chronically injured rat liver (CCl4 model).Methods: Mast cells were identified by histochemical and immunohistochemical methods, The immunoreactivity of liver and comparatively of rat peritoneal mast cells to the serpins alpha1-antitrypsin, alpha1-antichymotrypsin and antithrombin III was also studied.Results: In normal human and rat liver, mast cells were rarely found in portal tracts, and there was no change in cell numbers in nonfibrotic human or acutely injured rat livers, In contrast, cirrhotic human and rat livers contained numerous mast cells in the portal tracts and the fibrous septa, They exhibited strong immunoreactivity to the serpins, as did rat peritoneal mast cells.Conclusions: The results indicate that in the late stages of liver fibrogenesis, mast cells may be involved by displaying protease inhibitory activity in the fibrotic septa.