Meristem development and activity in gametophytes of the model fern, Ceratopteris richardii
Meristem development and activity in gametophytes of the model fern, Ceratopteris richardii
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DOI：
10.1016/j.ydbio.2018.10.005
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发表时间：
2018-12-15
影响因子：
2.7
通讯作者：
Gola, Edyta M.
中科院分区：
文献类型：
--
作者：
Bartz, Mateusz;Gola, Edyta M.
摘要水蕨是研究配子体发育过程及其调控的模式蕨类植物。成熟C. Richardii配子体的形成依赖于边缘分生组织的活动，但对分生组织的形成和结构的了解有限。因此，我们分析了伴随配子体发育的细胞事件，使用细胞谱系和增殖分析来解释边缘分生组织的建立和功能。我们证明：i）配子体在早期发育阶段缺乏顶端起始细胞或基于顶端细胞的分生组织; ii）预定形成边缘分生组织的细胞根据稳定模式分裂; iii）边缘分生组织中仅存在一个瞬时起始细胞，并且新的功能起始细胞的选择与其分裂的稳定序列有关。我们的研究结果有助于更好地了解的配子体生长和边缘分生组织的功能在水蕨的发展事件。在这项研究中建立的原则，使功能的初始细胞的鉴定，可以应用于分析的遗传和/或生理机制（S）管理分生组织维持维管植物，无论是在发育和进化的背景下。
Ceratopteris richardii is a model fern species widely used to analyze various developmental processes and their regulation in gametophytes. The form of mature C. richardii gametophytes depends on the activity of the marginal meristem, but knowledge on meristem formation and structure is limited. Therefore, we analyzed cellular events accompanying the development of gametophytes using cell lineage and proliferation analyses to explain the establishment and functioning of the marginal meristem. We show that: i) gametophytes are devoid of the apical initial cell or the apical cell-based meristem in the early developmental stages; ii) the cells that are predestined to form the marginal meristem divide according to a stable pattern; iii) only one transient initial cell is present in the marginal meristem, and the selection of a new functioning initial cell is related to a stable sequence of its divisions. Our results contribute to a better understanding of the developmental events underlying gametophyte growth and marginal meristem functioning in Ceratopteris. The principles, which were established in this study and enabled the identification of functioning initial cells, can be applied to analyze genetic and/or physiological mechanism(s) governing meristem maintenance in vascular plants, both in developmental and evolutionary contexts.