Assessment of Scanning Mobility Particle Sizer (SMPS) for online monitoring of delivered dose in an in vitro aerosol exposure system.
Assessment of Scanning Mobility Particle Sizer (SMPS) for online monitoring of delivered dose in an in vitro aerosol exposure system.
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DOI：
10.1016/j.tiv.2023.105650
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发表时间：
2023-07
期刊：
影响因子：
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通讯作者：
S. N. Shankar;Kiran Mital;Eric Le;Gregory S. Lewis;Arantzazu-Eiguren Fernandez;T. Sabo-Attwood;Chang-Yu Wu
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文献类型：
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作者：
S. N. Shankar;Kiran Mital;Eric Le;Gregory S. Lewis;Arantzazu-Eiguren Fernandez;T. Sabo-Attwood;Chang-Yu Wu
剂量学的实时监测对于减轻离线测量的限制至关重要。为了满足这一需求，使用扫描迁移率粒度仪（SMPS）来评估通过剂量计气溶胶体外吸入装置（DAVID）传递的剂量。CuO纳米颗粒悬浮在不同浓度（0.01-10 mg/mL）的乙醇中，使用Collison雾化器雾化，并以1:3（设置1）或1:18（设置2）的比例用空气稀释。从SMPS测量的气溶胶体积浓度、CuO浓度（6.4 g/cm3）、收集时间（5 ~ 30 min）、流速（0.5 LPM）和沉积面积（0.28 cm2）来看，质量剂量（DoseSMPS）随时间呈指数增长，范围为0.02±0.001 ~ 84.75±3.49 μg/cm2。电感耦合等离子体光学发射光谱（ICP-OES）测定的铜浓度（DoseICP）计算的剂量也随时间呈指数增长（0.01±0.01 ~ 97.25±1.30 μg/cm2）。在0.1 ~ 10 mg/mL范围内，doseicp_2与dosesmps_2的回归分析显示R2≥0.90。如图所示，SMPS可用于实时监测递送剂量，通过调整雾化器浓度、稀释空气和时间，在DAVID中可在≤30分钟内实现226倍范围的受控大剂量递送。
Real-time monitoring of dosimetry is critical to mitigating the constraints of offline measurements. To address this need, the use of the Scanning Mobility Particle Sizer (SMPS) to estimate the dose delivered through the Dosimetric Aerosolin VitroInhalation Device (DAVID) was assessed. CuO nanoparticles suspended in ethanol at different concentrations (0.01–10 mg/mL) were aerosolized using a Collison nebulizer and diluted with air at a ratio of either 1:3 (setup 1) or 1:18 (setup 2). From the aerosol volume concentrations measured by the SMPS, density of CuO (6.4 g/cm3), collection time (5–30 min), flow rate (0.5 LPM) and deposition area (0.28 cm2), the mass doses (DoseSMPS) were observed to increase exponentially over time and ranged from 0.02 ± 0.001 to 84.75 ± 3.49 μg/cm2. The doses calculated from the Cu concentrations determined by Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) (DoseICP) also increased exponentially over time (0.01 ± 0.01–97.25 ± 1.30 μg/cm2). Regression analysis between DoseICPand DoseSMPSshowed R2≥ 0.90 for 0.1–10 mg/mL. As demonstrated, the SMPS can be used to monitor the delivered dose in real-time, and controlled delivery of mass doses with a 226-fold range can be attained in ≤30 min in DAVID by adjusting the nebulizer concentration, dilution air and time.