Expression of rat fibroblast growth factor receptor 1 as three splicing variants during kidney development.
Expression of rat fibroblast growth factor receptor 1 as three splicing variants during kidney development.
复制标题
大鼠成纤维细胞生长因子受体 1 在肾脏发育过程中以三种剪接变体的形式表达。
DOI：
10.1152/ajprenal.1993.264.1.f66
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Ballermann,BJ
中科院分区：
文献类型：
--
作者：
Kim,EG;Kwon,HM;Burrow,CR;Ballermann,BJ
成纤维细胞生长因子（FGF）参与胚胎发生和血管生成过程。本研究旨在研究胚胎大鼠肾脏中FGF受体1 （FGFR-1）亚类的转录和转录后调控。利用鸡FGFR-1 cDNA探针对新生大鼠肾脏文库进行低严格筛选，克隆了两个全长FGF受体cDNA。测序显示，这些克隆的cdna是FGFR-1亚型的大鼠同源物，其中两个克隆代表FGFR-1的剪接变体β和γ。通过使用聚合酶链反应获得了肾脏表达第三种剪接变体（α）的证据。据预测，FGFR-1的剪接变体α和β分别产生具有三个和两个免疫球蛋白样结构域的细胞表面FGF受体，而γ -异构体可能代表该受体的细胞内形式。尽管这三种剪接变体在妊娠第14、17和20天的发育肾脏中都有表达，但在新生儿第1天和第7天以及成熟大鼠中，β -异构体的丰度远远大于α -和γ -异构体。Northern blot分析显示，与成熟肾脏相比，新生肾脏中FGFR-1的表达增强。我们得出结论，FGFR-1在肾脏中主要以β -异构体剪接变体的形式表达，并且该受体的表达在肾脏发育过程中增强。
Fibroblast growth factors (FGF) are known to participate in the processes of embryogenesis and angiogenesis. This study was undertaken to examine the transcriptional and posttranscriptional regulation of the FGF receptor 1 (FGFR-1) subclass in the embryonic rat kidney. Two full-length FGF receptor cDNAs were cloned using low-stringency screening of a neonatal rat kidney library with a chicken FGFR-1 cDNA probe. Sequencing revealed these cloned cDNAs to be rat homologues of the FGFR-1 subtype, with the two clones representing splicing variants beta and gamma of the FGFR-1. Evidence for renal expression of a third splicing variant (alpha) was obtained by use of the polymerase chain reaction. Splicing variants alpha and beta of FGFR-1 are predicted to produce cell-surface FGF receptors with three and two immunoglobulin-like domains, respectively, whereas the gamma-isoform may represent an intracellular form of the receptor. Although all three splicing variants were expressed in the developing kidney at days 14, 17, and 20 of gestation, at neonatal days 1 and 7 and in mature rats the beta-isoform was present in vastly larger abundance than alpha- and gamma-isoforms at all stages studied. Northern blot analysis revealed enhanced expression of FGFR-1 in the neonatal compared with the mature kidney. It is concluded that FGFR-1 is expressed in the kidney predominantly as the beta-isoform splicing variant and that expression of this receptor is enhanced during kidney development.